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Preparation and Screening of Protoplast Fusion Bacteria of Bacillus subtilis and Lactobacillus rhamnosus

Preparation and Screening of Protoplast Fusion Bacteria of Bacillus subtilis and Lactobacillus rhamnosus

Ye Ge1, Chunmiao Zhao1, Weitian Xie2, Ying Liu2,* and Chunhou Xu1,*

1College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang, Guangdong Province, China
2College of Food Science and Technology, Guangdong Ocean University, Zhanjiang, Guangdong Province, China

* Corresponding author: 406662864@qq.com; xuchunhou@sina.com

Fig. 1.

Growth curves of Bacillus subtilis and Lactobacillus rhamnosus. The incubation time was used as the abscissa, and the absorbance was used as ordinate to plot on the plot. The mid-logarithmic growth phase of these two strains was determined.

Fig. 2.

Fusion of the protoplast of the two strains; a, Protoplast of Bacillus subtilis; b, Protoplast of Lactobacillus rhamnosus; c, Fusion of the protoplast.

Fig. 3.

Acid production stability of the 5 regenerated bacterial strains.

Fig. 4.

Genetic stability of the 3 regenerated bacterial strains.

Fig. 5.

An image of growth on solid medium of three bacterial strains and a microscopy image of three bacterial strains (10×100): a and d were Bacillus subtilis, b and e were Lactobacillus rhamnosus and c and f were fusion of RH-3.

Pakistan Journal of Zoology

June

Vol. 53, Iss. 3, Pages 801-1200

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