Virus Elimination from Infected Garlic Plants Using Different Techniques
Virus Elimination from Infected Garlic Plants Using Different Techniques
S.A. Sidaros1; R.A. Omar; S.A. El-Kewey l and Samaa Abd El-Khalik2
ABSTRACT
Production of garlic virus free plant was attempted by means of chemotherapy, meristem-tip culture, and thermotherapy techniques. Three different meristem sizes (5, 3 and 1 mm long) were excised from three garlic cultivars (Chinese. Italian and Balady). Meristems were cultured on MS medium amended with 0.5 mg/L BA (Benzyle adenine). The best size for virus elimination and survival plants was 3 mm long whereas 5 mm long produced highest survival plants without virus elimination and I mm long failed to produce survival plants in two of the tested three cultivars. The suitable medium was MS + 0.5 mg/L BA that produced 33.3% survival plantlets whereas MS + 0.1 mg/L NAA + 0.5 mg/L BA and MS + 0.1 mg/L NAA produced 16.6 and 6.6% survival plantlets, respectively. Virazole (50 mg/L) was used as a virus inhibitor material in MS medium for culturing meristems (3 mm long) of cvs. Chinese and Balady. The produced plantlets were 100% virus free. Garlic cloves or two cultivars Balady and Chinese were treated with hot water and hot air at different terminated degrees of temperatures and periods. Treated cloves were planted in pots.
To share on other social networks, click on any share button. What are these?
