Polymorphism in the Exon 11 of Bovine Luteinizing Hormone Receptor Gene and its Association with Service per Conception in Pasundan Cows
Polymorphism in the Exon 11 of Bovine Luteinizing Hormone Receptor Gene and its Association with Service per Conception in Pasundan Cows
Syahruddin Said1*, Widya Pintaka Bayu Putra1 and Dani Nur Arifin2
Primer position (underline) and HhaI restriction site (5’...GCG||T...3’) in the bLHR gene (Bos indicus) according to GenBank: XM_01969469.1 with y=C/T.
The amplification product of bLHR gene on 1% agarose gel. M, marker (DNA ladder 100 bp); lanes 1-12, number of sample.
The fragments of bLHR gene using PCR-RFLP method with HhaI restriction enzyme on 2% agarose gel showed three genotypes of CC (155 bp), CT (303 bp and 155 bp) and TT (303 bp). M, marker (DNA ladder 100 bp).
Part of chromatogram showing characteristics of SNP at g.1553C>T in the exon 11 of Pasundan bLHR gene.
Part of chromatogram of new SNP at g.1626G>T in Pasundan bLHR gene in one individual sample.
Primer position (underline) and HhaI restriction site (5’...GCG||T...3’) in the bLHR gene (Bos indicus) according to GenBank: XM_01969469.1 with y=C/T.
Primer position (underline) and HhaI restriction site (5’...GCG||T...3’) in the bLHR gene (Bos indicus) according to GenBank: XM_01969469.1 with y=C/T.
