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Polymorphism Detection in Alpha-Lactalbumin Gene and its Association with Some Productive Traits in Egyptian Goats

Polymorphism Detection in Alpha-Lactalbumin Gene and its Association with Some Productive Traits in Egyptian Goats

Amira M. Nowier1, Hassan R. Darwish2, Sherif I. Ramadan3, Nadia A. Abo El-Maaty2 and Othman E. Othman2*

1Biotechnology Research Department, Animal Production Research Institute, Agriculture Research Center, Dokki, Giza, Egypt.
2Cell Biology Department, National Research Centre, Dokki, Egypt.
3Animal Wealth Development Department, Faculty of Veterinary Medicine, Benha University, Toukh, Egypt.
 
* Corresponding author: othmanmah@yahoo.com

Fig. 1.

Multiple sequence alignment of the first 14 nucleotides of α-LA gene exon III and their corresponding amino acids (NCBI accession No.MT163744.1 and MT163745.1) of this study with six α-LA reference sequences of goats retrieved from GenBank (NCBI accession No. KF781121.1 and KP940442.1 and DQ673921.1 and DQ629104.1 and M63868 and EU573193.1), Ovis aries (NCBI accession No. KY485140.1), Bubalus bubalis (NCBI accession No. KC415276.1) and Bos taurus (NCBI accession No. JN258330.1) species. P, Proline; V, Valine; H, Histidine; S, Serine; F, Phenylalanine; L, Leucine; D, Aspartic acid.

Fig. 2.

Neighbor-joining phylogenetic tree based on nucleotide sequences of α-LA gene exon III fragments of Egyptian goats (NCBI accession No.MT163744.1 and MT163745.1), with six α-LA reference sequences of goats retrieved from GenBank (NCBI accession No. KF781121.1 and KP940442.1 and DQ673921.1 and DQ629104.1 and M63868 and EU573193.1), Ovis aries (NCBI accession No. KY485140.1). Bubalus bubalis (NCBI accession No. KC415276.1) and Bos taurus (NCBI accession No. JN258330.1) species act as out-group.

Fig. 3.

PCR-SSCP conformation patterns of α-LA gene showing the two different patterns (genotypes) of Egyptian goat breeds; conformation pattern I represents genotype TT and conformation pattern II represents genotype TC.

Fig. 4.

Sequence chromatogram showing the α-LA gene exon III (268 bp) in Egyptian goat breeds. (a) Genotype TT; (b) Genotype TC, * means the start of exon III.

Pakistan Journal of Zoology

August

Vol. 54, Iss. 4, Pages 1501-2001

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