Chun-Yi Lin1, Meng-Ling Wu2, Tang-Long Shen1, Hsin-Hung Yeh3, Ting-Hsuan Hung1*
...n: justify; ">Two citrus viroids, Citrus exocortis viroid (CEVd) and Hop stunt viroid (HSVd), have been reported decades ago without further pathological and genetic researches in Taiwan. Here, bioassay, multiplex RT-PCR and multiplex real-time RT-PCR were used to detect the current status of the two viroids showing HSVd was more prevalent than CEVd and ...
Elharony, S.B.1,3, SaharA.Youssef2 and Richard F. Lee3
...>DNA and RNA viruses and viroid provides a new approach to virus
and virus like detection and identification. The method provides a
pure preparation of un-degraded RNA and DNA in high yield and
can be completed within 2 h. It is particularly useful for processing
large number of samples and for isolation of RNA and DNA from
small quantities of tissues. The method does not require expensive
E. K. Allam1; Kh. A. El-Dougdoug1; M. A. Amer2; A. A. Abou-Zaid2; and S. M. Amin2
...y;">Potato spindle tuber viroid (PSTVd) was detected in potato plants grown in Egypt using return-polyacrylamide gel electrophoresis (R-PAGE) and molecular techniques. The method involved the extraction of circular nucleic acids followed by electrophoretic analysis of the extracts on 5% polyacrylamide gel. A reverse transcription-polymerase Chain reaction (RT-PCR) assay was used for the isolation and identification of PSTVd cDNA from infected plant materials u...
El-Dougdougi, Kh. A.; Dawoud2, Rehab A.; Rezk2, A.A. and Sofy3, A.R.*
...n peach trees. The three viroids; CEVd, HSVd and PLMVd were detected frequently in sweet orange and peach occasionally in grapevine, pear, mango, plum and apricot in Egypt....
Sofyl *, A.R.; Soliman , A.M.; Mousal, A.A. and El-Dougdoug , Kh.A.
...cterization of Hop stunt viroid which has been isolated previously in Egypt from sweet orange infected with gummy bark disease namely Citrus viroid Il. The native structure of mature, circular forms of the gummy bark agent was detected by gel electrophoresis. The expected size of amplified cDNA by RT-PCR was approximately 300 bp. A phylogenetic tree of the Egyptian citrus gummy bark agent (Accession no. FJ984562) revealed 10...
...om development of citrus viroids. The geographical distribution of the gummy bark disease in some world countries depend on high temperatures for viroid-symptom expression. So, correlation between climatic factors and gummy bark disease through GIS is studied. We designed a satellite map for the gummy bark disease distribution all over the world using the previous registered results. Superimposed maps of BIOCLnvf annual Mint...
El-Dougdoug, Kh.A.; Rezk , A.A.; Dawoud, Rehab A. and Sofy, A.R.
...fy;">Chrysanthemum stunt viroid Egyptian isolate (CSVd-EG) was isolated from infected Chrysanthemum plants. It is a member of Pospiviroidae. In order to study the structure of CSVd-EG, it was reverse transcribed in total RNA from infected leaves and then amplified by polymerase chain reaction (PCR) using Pospiviroid-CCR specific primers. Purified gel RT-PCR product (⁓199) was cloned into...
Nasr-Eldin1 , M. A.; El-Dougdoug2, K. A.; Othman2, B. Ahmedi , Sabah A, and Abdcl-Azizl , S. Il.
...t-align: justify;">Three viroids: Citrus exocorlis viroid (CEVd), Hop stunt viroid (HSVd), and Potato spindle tuber viroid (PSTVd) can be detected in naturally infected grapevine by biological indexing and molecular methods. In the present study, direct tissue-printing and dot-blot on the membranes, procedures has been applied on a large scale for an ini...
ZARYAB KHALID SIAL & FARAH KHAN
... of potato spindle tuber viroid (PSTVd) on potato plants in Pakistan. RNA was extracted and purified using optimized Trizol reagent and RNeasy Miniplant Kit. The RNA obtained was converted into cDNA on the same day and amplified. The identification of PSTVd was carried out by Sequencing and BLAST. The advanced optimized Rapid Trizol reagent method was comparatively found best and reproducible as compared to the second method. The Sequencing and BLAST confirmed...
