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Partial Characterization of α-amylase Produced from Aspergillus niger using Potato Peel as Substrate

Partial Characterization of α-amylase Produced from Aspergillus niger using Potato Peel as Substrate

Shahzad Mahmood1, Memuna Ghafoor Shahid1, Muhammad Irfan2, Muhammad Nadeem3*, Quratulain Syed3

1Department of Botany, Government College University, Lahore, Pakistan
2Department of Biotechnology, University of the Sargodha, Sargodha, Pakistan
3Food & Biotechnology Research Center, Pakistan Council of Scientific & Industrial Research (PCSIR) Laboratories Complex, Ferozpure Road, Lahore, Pakistan.

*Corresponding author Dr. Muhammad Nadeem, [email protected] 

 

Figure 1:

Optimum incubation period for α-amylase activity (LSD 5%= 92.63).

Figure 2:

Optimum incubation temperature for α-amylase activity (LSD 5%= 125.66).

Figure 3:

Effect of pH on α-amylase activity and stability (LSD 5%= 156.06).

Figure 4:

Effect of substrate concentrations on α-amylase activity.

Figure 5:

Effect of different metal ions and chelator on α-amylase activity.

Punjab University Journal of Zoology

June

Vol.39, Iss. 1, Pages 01-134

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