Oral Liposome Vaccines Containing Cyprinid herpesvirus 3 (CyHv3) and Aeromonas hydrophila Antigen for the Immunization of Common Carp Cyprinus carpio
Oral Liposome Vaccines Containing Cyprinid herpesvirus 3 (CyHv3) and Aeromonas hydrophila Antigen for the Immunization of Common Carp Cyprinus carpio
Sundus Oun Ali Al-Zaini1, Mohanad A. Al-Bayati3*, Khazaal Abbas Khazaal2, Salma Talib Salih1
Experimental design.
Experimental design.
The light micrograph of liposome KHV and A. hydrophila show the lamellar of liposome with clusters of liposomes of multivesicles form and in otherwise denoted to the general profile of loading incidence and empty form. Further, the signs of inclusive aggregation of liposome KHV and A. hydrophila behavior trend to signify a crowed grab form.
The Osmo-tolerance in NaCl challenge solution of (A) liposome KHV and (B) A. hydrophila at zero time and after one hour.
The pH tolerance of (A) liposome KHV and (B) A. hydrophila in an Acid-Base challenge solution.
Mortality in groups (immunized fish in good quality water by oral vaccination Containing 0.63 µg KHV Liposome vaccine /fish/day for five days (1.1, 1.2٭) and unvaccinated fish groups in good quality water (1.5, 1.6) and Mortality in groups (immunized fish in bad quality water by oral vaccination Containing 0.63 µg KHV Liposome vaccine/fish/day for five days(1.3, 1.4٭) and unvaccinated fish groups in bad quality water (1.7, 1.8). ٭Significantly difference from control (p≤0.05).
Mortality in vaccination fish for 5 days Aeromonas hydrophila liposome vaccine at dose 1*109 bacteria/fish/day pound (2.1, 2.2) and unvaccinated fish pound (2.5, 2.6) was challenged by intramuscular injection with A. hydrophila at 3*108 CFU/fish, Mortality in vaccination fish for 5 days Aeromonas hydrophila liposome vaccine at dose 1*109 bacteria/fish/day pound(2.3,2.4) and unvaccinated fish pound (2.7, 2.8) was challenged by intramuscular injection with A. hydrophila at 3×107 CFU /fish. *Significant difference between vaccinated and control at level (p≤0.05).
