Molecular Sequencing Analysis of Fasciola Spp. in Sheep
Molecular Sequencing Analysis of Fasciola Spp. in Sheep
Israa M. Essa*, Ghazi Y. Azzal, Alaa Tariq Abdulwahid
Figure 1:
Electrophoresis of some PCR products targeting 18S rRNA gene of Fasciola;Lane (M): Ladder marker (100-1500 bp); Lanes (1, 2, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14 and 15): Positive samples; Lanes (3 and 4): Negative samples; Lane (N): Negative control.
Figure 2:
Multiple sequence alignment of local and NCBI-GenBank Fasciola isolates showing nucleotide similarity and substitution mutations at 18S rRNA gene.
Figure 3:
Phylogenetic tree analysis of local and NCBI-GenBank Fasciola isolates showing the level of identity at 99.72-99.78%.
