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Molecular Sequencing Analysis of Fasciola Spp. in Sheep

Molecular Sequencing Analysis of Fasciola Spp. in Sheep

Israa M. Essa*, Ghazi Y. Azzal, Alaa Tariq Abdulwahid

Department of Parasitology, College of Veterinary Medicine, University of Basrah, Basra, Iraq.

 
*Correspondence | Israa M. Essa, Department of Parasitology, College of Veterinary Medicine, University of Basrah, Basra, Iraq; Email: [email protected]

Figure 1:

Electrophoresis of some PCR products targeting 18S rRNA gene of Fasciola;Lane (M): Ladder marker (100-1500 bp); Lanes (1, 2, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14 and 15): Positive samples; Lanes (3 and 4): Negative samples; Lane (N): Negative control.

Figure 2:

Multiple sequence alignment of local and NCBI-GenBank Fasciola isolates showing nucleotide similarity and substitution mutations at 18S rRNA gene.

Figure 3:

Phylogenetic tree analysis of local and NCBI-GenBank Fasciola isolates showing the level of identity at 99.72-99.78%.

Advances in Animal and Veterinary Sciences

December

Vol. 12, Iss. 12, pp. 2301-2563

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