1Depatment of Biotechnology, College of Science, University of Baghdad, Iraq
2Agriculture College, Al-Qasim Green University, Iraq
3College of Environmental Sciences, Al-Qasim Green University, Iraq
Corresponding author: Hashim Hadi Al-Jebory
hashimhadi@agre.uoqasim.edu.iq
ABSTRACT
This study was carried out to assess the influence of in-ovo injection of broiler embryo with Zinc-methionine, on some characteristics of biochemistry of blood serum in addition to total count of some bacteria in the gut of broiler. A 300 of hatching eggs of Ross broiler breeder flock were divided randomly into 4 treatment groups, 75 eggs in each group, which were: (control) non-injected, T2 injected with 60 ppm, T3 injected with 80 ppm and T4 injected with 100 ppm respectively. After hatching 45 chicks from were chosen each treatment and divided randomly into 3 replicates and reared until 35 day age. Results found that, there were significant (P<0.01) effects of T3 and T4 in the total count of lactobacillus in the illume and jejunum, whereas T1 was significantly higher treatment in the E. coli at age of 21 and 35 day. Also T4 was the highest treatment in the lactobacillus at day 35 of the age of the illume and jejunum. For IgG there was significant (P<0.01) effects of T4, also T3 and T4 were affected the levels of IgA significantly (P<0.01), also IgM level was significant (P<0.01) at higher concentration in the T2 in front of other treatments. Total protein was affected by the T2 and T3 which was higher in these treatments in comparative with other treatments significantly (P<0.01). all treatments were at low level of glucose when comparing with T1 which was at higher concentration significantly (P<0.01). in conclusion, zinc-methionine injection has enhance the immunity status of birds and improve the gut microbiota into positive way.
Novelty Statement | This study is the first to use zinc-methionine to enhance the intestinal microbiota of broilers in Iraq-Babylon.
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