Molecular Identification and Genetic Diversity of Notopterus notopterus (Pallas, 1769) Using Mitochondrial COI Gene and RAPD Markers in Riverine Water of Pakistan
Molecular Identification and Genetic Diversity of Notopterus notopterus (Pallas, 1769) Using Mitochondrial COI Gene and RAPD Markers in Riverine Water of Pakistan
Waqas Ahmad*, Muhammad Naeem
Sampling site and study area of Notopterus notopterus fish of Pakistan.
Notopterus notopterus fish identified in present study (Lateral view).
Maximum likelihood tree analyses of Notopterus notopterus identified from Pakistan with Notopterus notopterus reported from neighbour countries (India, Bangladesh and Indo-mayanmar). The node values represent the confidence level of 2000 bootstrap replicates using MEGA-X.
DNA fingerprints in N. notopterus of Chenab, Satluj, Ravi, Jehlum and Indus using 5 RAPD markers A OPA-02. B OPA-05. C OPA-07. D OPA-11. E OPA-16. Samples C1-C3 for Chenab, S1-S3 for Satluj, R1-R3 for Ravi, J1-J3 for Jehlum, I1-I3 for Indus River for each primers and M is molecular marker (Ladder).
UPGMA dendrogram based on RAPD polymorphic data showed genetic connectivity among riverine populations of Ravi, Satluj, Jehlum, Indus and Chenab.
RAPD diversity analysis of five population of N. notopterus.
