Molecular Identification and Characterization of Fruit Flies of Genus Bactrocera (Diptera: Tephritidae) in Pakistan
Molecular Identification and Characterization of Fruit Flies of Genus Bactrocera (Diptera: Tephritidae) in Pakistan
Jam Nazeer Ahmad1*, Taiba Sharif3, Samina J.N. Ahmad2, Sumaira Maqsood4 and Fariha Zafar3
Multiple alignments of DNA sequences through CLUSTAL OMEGA from DNA amplification of 3 Pakistani Bactrocera species, B. cucurbitae 180319-003 (MK296116) isolate FSD-03, B. dorsalis 180319-004 (MK296118)isolate FSD-18 and B. zonata 180319-016 (MK296117) isolate FSD-16 with NCBI GenBank sequences, B. Cucurbitae (KJ753953.1), B. dorsalis (KF998634.1) and B. zonata (KP851002.1)
Phylogenetic analysis by Maximum Likelihood Method produced from DNA sequences of 3 Bactrocera species using mitochondrial Cytochrome oxidase I (mCOI) gene based primers. (A) B. cucurbitae 180319-003 (MK296116) (B) B. zonata 180319-016 (MK296117) (C) B. dorsalis 180319-004 (MK296118)