In Vitro Effectiveness of Silver Nanoparticles against Root-Knot Nematode (Meloidogyne incognita)
In Vitro Effectiveness of Silver Nanoparticles against Root-Knot Nematode (Meloidogyne incognita)
Khansa Nazir1,*, Tariq Mukhtar1 and Humayun Javed2
ABSTRACT
As the use of nematicides is becoming prohibitive in many countries due to their detrimental consequences, the management of plant parasitic nematodes using nanoparticles can be one of the important alternatives. In the present study, the nematicidal activity of silver nanoparticles (AgNP) was investigated against the most destructive root-knot nematode (Meloidogyne incognita). The maximum mortality of juveniles was recorded at a concentration of 100 mg/ml followed by 75 mg/ml of AgNP. The minimum mortality was recorded with 25 mg/ml of AgNP. With the increase in concentration, there was a corresponding increase in the mortality of juveniles showing a direct relationship between mortality and concentration of nanoparticles. The effect of time on mortality was also found significant. With the increase in time, there was a corresponding increase in mortality and the relationship was found to be directly proportional. Similarly, the maximum hatching inhibition of M. incognita eggs was recorded at a concentration of 100 mg/ml of nanoparticles followed by 75 mg/ml of AgNP. The minimum inhibition was recorded with 25 mg/ml of AgNP. It was found that with the an increase in concentration, there was a corresponding increase in hatching inhibition showing direct relationship between hatching inhibition and concentrations of the nanoparticles. Similarly, the maximum hatching inhibitions were recorded after 6th day followed by 5th day. The hatching inhibition was found directly proportional to time duration as it increased with an increase in number of days. It is concluded from the present study that AgNP possess nematicidal activity against root-knot nematodes and can act as an alternative to high-risk synthetic nematicides or inconsistent biological control agents without causing any phytotoxicity.
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December 2019
Vol. 51, Iss. 6, Pages 1999-2399
