Improvement of Mouse Parthenogenetic Blastocyst as Primary Colony Source of Parthenogenetic Embryonic Stem Cell (pESC) using CHIR99021
Improvement of Mouse Parthenogenetic Blastocyst as Primary Colony Source of Parthenogenetic Embryonic Stem Cell (pESC) using CHIR99021
Dwi Budiono1,5, Ratih Rinendyaputri2, Ariyani Noviantari2, Mokhamad Fahrudin3, Ni Luh Putu Ika Mayasari4, Vista Budiariati1,6, Diah Nugrahani Pristihadi1,3, Noer Muhamad Dliyaul Haq1, Arief Boediono3*
Figure 1
Immunocytochemistry staining of the 7th day primary colonies of culture. (A) Immunocytochemistry positive. (B) Immunocytochemistry negative. Bar = 25µm.
Figure 2
Alkaline phosphatase (ALP) staining of primary colony on 7th day of culture. (A) Control; (B) CHIR99021 0 mmol/l; (C) CHIR99021 0.003 mmol/l group. Blue arrow: weak ALP expression; Clue circle: No ALP expression. Bar = 25µm.
May 2022
Vol. 10, Iss. 5, Pages 955-1188
