Submit or Track your Manuscript LOG-IN

Identification of Nuclear Factor-κB Pathway Genes in Chicken Erythrocytes and their Expression Level in Erythrocytes after Infection with Mycoplasma synoviae

Identification of Nuclear Factor-κB Pathway Genes in Chicken Erythrocytes and their Expression Level in Erythrocytes after Infection with Mycoplasma synoviae

Afrasyab Khan1,3, Ali Raza Jahejo1,3, Meng-li Qiao1,3, Xin-yu Han1,3, Raza Ali Mangi1,3, Ding Zhang1,3, Yu-hai Bi2, George F Gao1,3* and Wen-xia Tian1,3*

1College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, China
2CAS Key Laboratory of Pathogenic Microbiology and immunology, Collaborative Innovation    Center of Infectious Diseases, Institute of Microbiology, Center for Influenza Research and Early-Warning (CASCIRE), Chinese Academy of Science, Beijing 100101, China 
3Shanxi Key Laboratory of Protein Structure Determination, Shanxi Academy of Advanced Research and Innovation, Taiyuan 030032, China
 
*   Corresponding author: gaof@im.ac.cn, wenxiatian@126.com

Fig. 1.

Transmission electron Microscopy (TEM) of chicken erythrocytes showing interaction with M. synoviae. Magnification 20000 × or 10000 ×.

Fig. 2.

The expression pattern of NF-κB signaling pathway and other immune system genes in chicken erythrocytes on 0 h, 2 h, 6 h, 10 h post-infection (Hpi).. This experimental study group was as follows: The control group (Con, erythrocytes treated with PBS, n = 3) and the Experiment group (Exp, erythrocytes infected with M. synoviae, n = 3). Expression levels of NF-κB signaling pathway and other immune system genes were relatively calculated to that of 18S rRNA the housekeeping genes via quantitative real-time PCR. SEM (standard error of mean) represented by error bars. Bars with asterisks indicate a significant (*P < 0.05, **P < 0.01, ***P ≤ 0.001) Up or Downregulated relative mRNA expression when compared to uninfected control group.

Pakistan Journal of Zoology

February

Pakistan J. Zool., Vol. 56, Iss. 1, pp. 01-501

Featuring

Click here for more

Subscribe Today

Receive free updates on new articles, opportunities and benefits


Subscribe Unsubscribe