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A New Species of the Genus Otacilia

Otacilia dadongshanica sp. nov., male holotype.

A New Species of the Genus Otacilia

Otacilia dadongshanica sp. nov., male holotype.

Identification of Nuclear Factor-κB Pathway Genes in Chicken Erythrocytes and their Expression Level in Erythrocytes after Infection with Mycoplasma synoviae

Identification of Nuclear Factor-κB Pathway Genes in Chicken Erythrocytes and their Expression Level in Erythrocytes after Infection with Mycoplasma synoviae

Afrasyab Khan1,3, Ali Raza Jahejo1,3, Meng-li Qiao1,3, Xin-yu Han1,3, Raza Ali Mangi1,3, Ding Zhang1,3, Yu-hai Bi2, George F Gao1,3* and Wen-xia Tian1,3*

Fig. 1.

Transmission electron Microscopy (TEM) of chicken erythrocytes showing interaction with M. synoviae. Magnification 20000 × or 10000 ×.

Fig. 2.

The expression pattern of NF-κB signaling pathway and other immune system genes in chicken erythrocytes on 0 h, 2 h, 6 h, 10 h post-infection (Hpi).. This experimental study group was as follows: The control group (Con, erythrocytes treated with PBS, n = 3) and the Experiment group (Exp, erythrocytes infected with M. synoviae, n = 3). Expression levels of NF-κB signaling pathway and other immune system genes were relatively calculated to that of 18S rRNA the housekeeping genes via quantitative real-time PCR. SEM (standard error of mean) represented by error bars. Bars with asterisks indicate a significant (*P < 0.05, **P < 0.01, ***P ≤ 0.001) Up or Downregulated relative mRNA expression when compared to uninfected control group.

Pakistan Journal of Zoology

December

Pakistan J. Zool., Vol. 56, Iss. 6, pp. 2501-3000

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