Expression Patterns of BMP15 Gene in Folliculogenesis of Buffalo (Bubalus bubalis)
Jinfeng Liu1,2, Yanhong Cao3, Tong Feng1, Laiba Shafique1, Chan Luo1, Peng Zhu1,4,* and Qingyou Liu1,*
RT-PCR results for the buffalo BMP15 gene. A: Lane M, marker III; Lane 1-2, RT-PCR product; B: Lane M1, supercoiled DNA ladder; Lane 1-2, pMD-18T-BMP15.
Buffalo BMP15 nucleotide and amino acid sequences.
Multiple alignment and analysis of deduced amino acid sequence of BMP15.
Neighbor-Joining phylogenetic tree based on the BMP15 protein among some species.
The protein domain of BMP15.
The secondary structure of buffalo BMP15 protein.
The tertiary structure of buffalo, bovine and homo BMP15 protein.
Agarose gel electrophoresis of PCR product of buffalo ALK6 in different tissues. 1, skin; 2, bone; 3, granular cells; 4, cumulus cells; 5, ovary; 6, testis; 7, muscle; 8, genital ridge; 9, heart; 10, liver; 11, lung; 12, kidney; 13, brain; 14, hypophysis; 15, hypothalamus; 16, water.
The expression pattern of BMP15 during buffalo folliculogenesis and embryogenesis.
BMP15 immunoreactivity in the different structures found within buffalo ovaries and testis. A, Primordial follicle in genital ridge of foetus between 60-90 days post concep tion (dpc); B, Primordial follicle in ovary of foetus; C, Primordial follicle in ovary of adult buffalo; D, primary follicle; E, secondary follicle; F, small antral follicle; G, COC of a large antral follicle; H, testis of foetus; I, testis of adult buffalo; J, negative control of testis; K, negative control of ovary. Cyst, primordial cyst; O, oocyte; GC, granulosa cells; CC, cumulus cells; MGC, mural granulosa cells. Scale bars represent 20 μm.