Evaluation of the Anti-Coccidial Effect of Cashew Oil and/or Toltrazuril in Chickens Experimentally Infected with Eimeria tenella
Evaluation of the Anti-Coccidial Effect of Cashew Oil and/or Toltrazuril in Chickens Experimentally Infected with Eimeria tenella
Kamal Ahmed El-Shazly1, Amira Abd El-Latif1, Nagwa Elhawary2, Asmaa F. Alswaf1, Foad Farrag3, Samy Sayed4, Mohamed El-Sharnouby5 and Mustafa Shukry6*
Histological structure of intestine (cecum) showing normal both covering epithelium and intestinal crypts (arrow) at 9th and 23th dpi., in non-infected non-treated chicken (G1), heavily infestation of the intestinal crypts with different coccidial stages (arrow) at 9th dpi. and dead coccidial stages (arrow) at 23th dpi., in infected non treated chicken (G2), marked decrease of affected crypts which contained the schizont of the parasite (arrow) and associated with intestinal hyperplasia (arrowhead) at 9th dpi. and hyperplasia of the crypts lining epithelium (arrow) with mild interstitial mononuclear cells infiltration (arrow) at 23th dpi., in infected chicken treated with cashew oil (G3), marked decrease of the number of live coccidial schizont stages (arrow) at 9th dpi. and hyperplasia of the crypts lining epithelium (arrow) with slight interstitial mononuclear cells infiltration at 23th dpi., in infected chicken treated with toltrazuril (G4), normal mucosa with apparent dead parasitic stages within the intestinal epithelial cells (arrow) at 9th dpi. and slight hyperplasia of the crypts lining epithelium (arrow) at 23th dpi., in infected chicken treated with cashew oil + toltrazuril (G5), H & E, X200.
Histological structure of liver showing normal hepatocytes (arrow) at 9th and 23rd dpi., in non-infected non treated chicken (G1), necrotic foci associated with mononuclear cells infiltration (arrow) 9th dpi. and a moderate degree of fat hepatic vacuolation (arrow) at 23rd dpi., in infected non treated chicken (G2), periportal eosinophilic cells infiltration (arrow) at 9th dpi. and a mild degree of hepatic vacuolation (arrow) at 23rd dpi., in infected chicken treated with cashew oil (G3), small area of periportal eosinophilic cells infiltration (arrow) at 9th dpi. and normal hepatocytes (arrow) at 23rd dpi., in infected chicken treated with toltrazuril (G4), a mild degree of mononuclear and eosinophilic cells infiltration (arrows) at 9th dpi. and normal hepatocytes (arrow) at 23rd dpi., in infected chicken treated with cashew oil + toltrazuril (G5), H & E, X200.
Histological structure of kidney showing normal renal glomeruli and tubules (arrowhead and arrow, respectively) at 9th and 23rd dpi., in non-infected non treated chicken (G1), features of interstitial nephritis (arrow indicates mononuclear infiltration) at 9th dpi. and features of regenerative tubular basophilia (arrow) at 23rd dpi., in infected non treated chicken (G2), foci of interstitial nephritis (arrow indicates mononuclear infiltration) at 9th dpi. and mild degenerative changes within the renal tubular epithelium (arrow) (arrowhead indicates normal glomerulus) at 23rd dpi., in infected chicken treated with cashew oil (G3), mild degenerative and desquamative changes within the renal tubules (arrow) at 9th dpi. and normal renal glomeruli (arrowhead) and tubules (arrow) at 23rd dpi., in infected chicken treated with toltrazuril (G4), mild cloudy swelling of the renal tubules (arrow) (arrowhead indicates normal glomerulus) at 9th dpi. and normal renal glomeruli (arrowhead) and tubules (arrow) at 23rd dpi., in infected chicken treated with cashew oil+toltrazuril (G5). H & E, X200.
