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Evaluating the Impact of Various LED Light Spectrums on Dendrobium officinale Tissue Culture Seedlings

Evaluating the Impact of Various LED Light Spectrums on Dendrobium officinale Tissue Culture Seedlings

Hafiz Ishtiaq Ahmad1, Jinlong Zhang1, Fuxun Luo1, Owais Iqbal2 and Yuying Wang1*

1College of Landscape and Horticulture, Yunnan Agricultural University, Kunming 650201, China; 2State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming 650201, China.

 
*Correspondence | Yuying Wang, College of Landscape and Horticulture, Yunnan Agricultural University, Kunming 650201, China; Email: wyysxp@126.com

Figure 1:

Dendrobium officinale tissue culture seedlings were illuminated under different LED light qualities condition. Different letters indicate various color of light spectrum.

Figure 2:

Dendrobium officinale tissue culture seedlings were irradiated under different light sources, including red, blue, yellow, green, combined red and blue (RB), mixture of red, blue, and yellow (RBY), a combination of red, blue, and green (RBG), a blend of red, blue, and white (RBW) compared with fluorescent white light (CK)

Figure 3:

Soluble sugar content, soluble protein content, and root activity in tissue culture seedlings of D. officinale under different light qualities. Different letters (a, b, c, d, e, f) indicate significant differences between treatments at P≤0.05 by using Duncan’s test. Vertical bars represent the standard deviation of the means (n = 10) in soluble sugar and protein, whereas the standard deviation of the means (n = 6) in root activity.

Figure 4:

Chlorophyll a, chlorophyll b, total chlorophyll, and Chl a/Chl b ratio in tissue culture seedlings of D. officinale under different light qualities. Different letters (a, b, c, d, e, f, g) indicate significant differences between treatments at P≤0.05 by using Duncan’s test. Vertical bars represent the standard deviation of the means (n = 10).

Figure 5:

Superoxide dismutase, peroxidase, catalase, and malondialdehyde enzyme activity in tissue culture seedlings of D. officinale under different light qualities. Different letters (a, b, c, d, e, f, g) indicate significant differences between treatments at P≤0.05 by using Duncan’s test. Vertical bars represent the standard deviation of the means (n = 10) in superoxide dismutase, peroxidase, and catalase whereas the standard deviation of the means (n = 6) in malondialdehyde.

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Pakistan Journal of Zoology

June

Pakistan J. Zool., Vol. 56, Iss. 3, pp. 1001-1500

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