Effects of Grp78-Knockout on the Functionalities of Goat Trophoblast Cells
Effects of Grp78-Knockout on the Functionalities of Goat Trophoblast Cells
Lili Zhao1, Fan Zhao1 and Yaping Jin2*
ABSTRACT
As the main components of the outermost membrane during embryonic development, trophoblast cells play an important role in embryo implantation and placenta formation. However, the molecular mechanism of trophoblast cell proliferation, invasion and hormone secretion remains elusive. In this study, we explored the role of GRP78 in the functionalities of goat trophoblast cells (GTCs). The Grp78 gene was efficiently knockout by using the CRISPR/Cas9 system, which resulted in altered morphology and function of GTCs. The cell shape showed a subrounded configuration, and the cell size also significantly increased. Furthermore, Grp78 knockout significantly decreased proliferation and adhesion activity, while increasing invasion activity. The secretion of estradiol and progesterone was also dramatically decreased after Grp78 knockout. Our results strongly suggest that GRP78 plays an important role in maintaining the normal functionalities of GTCs.
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