Effect of Fasudil Hydrochloride and H2 on the Post-Thaw Viability of Cryopreserved Porcine Adipose-Derived Stem Cells
Effect of Fasudil Hydrochloride and H2 on the Post-Thaw Viability of Cryopreserved Porcine Adipose-Derived Stem Cells
Maoying Zhu, Yuntao Ji, Xiaoyu Wang, Junying Pu and Changqing Qu*
Morphological characteristics of ADSCs cultured for 24 h after thawing. A, control group (CK); B, GSH group (100×); C, FH group (100×); D, GSH + FH group (200×).
Morphological characteristics of ADSCs cultured for 24 h after thawing in which the cryopreservation solution contained H2. A, control group; B, GSH group (100×); C, FH group (100×); D, GSH + FH group (200×).
Values of MTT of post-thawed ADSCs treated with GSH and FH. Data are shown as mean values ± SD (n=3). *p<0.05 compared with the control. Control (CK), GSH (100 µM GSH group), FH (10 µM FH group) and GSH + FH (100 µM GSH+10 μM FH group).
Values of MTT of post-thawed ADSCs treated with GSH, FH and Hydrogen gas. Data are shown as mean values ± SD (n=3). *p<0.05 compared with the control. Control (CK), GSH (100 µM GSH group), FH (10µM FH group) and GSH+FH (100 µM GSH+10μM FH group).
Analysis of apoptosis in post-thaw cells by flow cytometry. A, conventional cryopreservation solution (control); B, cryopreservation solution containing GSH; C, cryopreservation solution containing FH; D, cryopreservation solution containing GSH and FH.
