Effect of Different Dietary Protein Levels on Egg Development and its Response to Inducing Agents during Induced Spawning of Channa marulius
Effect of Different Dietary Protein Levels on Egg Development and its Response to Inducing Agents during Induced Spawning of Channa marulius
Muhammad Hafeez-ur-Rehman1, Farzana Abbas1, Muhammad Ashraf1, Naeem Tariq Narejo2, Khalid Javed Iqbal3, Ghulam Abbas4* and Syedah Andleeb5
ABSTRACT
Brood stock of Channa marulius with an average weight of 948.02±5.72g was randomly stocked in duplicate earthen ponds (90×70×4ft) and fed on 40%, 35% and 30% protein diet @5% of their live body weight. In 40% protein diet treatment, male fish was injected 1st dose with ovaprim + HCG (0.3+0.3ml) and female fish was given 2nd dose after 24 hrs of intervals with ovaprim (0.2ml), while 1st dose ovaprim (0.7ml)+HCG (1.0ml) and 2nd dose ovaprim (0.7ml) was given to the females. The treatment which was given 35% protein diet received 1st HCG+HMG (0.3+0.3ml) and ovaprim (0.2ml) to males and ovaprim (0.5ml)+fresh PG (1.0 ml), 2nd dose ovaprim (0.7ml) and 30% protein containing feed treatment 1st dose with ovaprim+HMG (0.3+0.3ml) and 2ndovaprim (0.2ml) to males and ovaprim (0.3ml) + HMG (1.0ml) with 2nd dose ovaprim (0.7ml kg-1) to females were injected. The highest average fecundity with latency period of 47.70±0.54 h was observed in treatment 3 (40% CP) while treatment 2 (35% CP), in combination of ovaprim + fresh PG showed very short latency period when compared to the ovaprim+HCG injected fish. Treatment 1 and control failed to spawn. Fish fed on 30% protein diet (treatment 1) and the control had the lowest fecundity showing insufficiency of protein required for proper development of the ovary. These studies revealed that 40% protein diet not only improved fish growth and health but also enhanced egg fecundity, gonadal development and spawning.
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