Differential Expression of the KIT Gene in Liaoning Cashmere Goats with different Coat Colors
Differential Expression of the KIT Gene in Liaoning Cashmere Goats with different Coat Colors
Jianping Li1, Qian Jiang2, Wei Chen2, Yumei Li3, Huaizhi Jiang4, Jinlong Huo5 and Qiaoling Zhang2*
A, The single mutation of KIT-exon13 in cashmere goats with different coat color. (I) Sequencing of KIT-exon13 in dominant white goat. (II) Sequencing of KIT-exon13 in dominant black goat. (III) Case showing nt 491 A→G missense mutation in white and black goat; B, Alignment of KIT-exon13 amino acid sequence prediction of cashmere goats with other mammals; C, The prediction of secondary structure in white cashmere goat and black cashmere goat. (I) The prediction of goat KIT-exon13 amio acid at the hydrophobic and the secondary structure. The unit of abscissa is amino acids digits and the ordinate is the value of the hydrophilic and hydrophbic balance(HLB). (II) The prediction of base secondary structure in goats. (III) Phylogenetic tree of KIT-exon13 gene of goats with different coat color.
A, Quantifications of KIT-exon13 in white cashmere goats and black cashmere goats; B, Western Blot analysis of c-KIT protein expression in skin samples with different coat color; C, H&E staining analysis of the expression of KIT-13 in skin samples about fair follicle and hair bulb for goats with different coat color. (a and c) the white goats and the black goats KIT-exon 13 expression (100×). (b and d) the white goats and the black goats KIT-exon 13 expression (200×). The number of hair bulbs analysis of c-KIT expression in skin samples with different coat color. ORS, outer root sheath; IRS, inner root sheath; HS, hair shaft cells; D, (I) Representative immunohistochemical staining of KIT in fair follicle for cashmere goats with different coat color. (a and b) the white goat and the black goat KIT-exon 13 anbidy expression (100×). (c and d) the white goat and the black goat KIT-exon 13 nucleus expression (100×). (e and f) the white goat and the black goat KIT-exon 13 anbidy expression (200×). (g,h) the white goat and the black goat KIT-exon 13 nucleus expression (200×). (II) The percentage of KIT-13 in different coat color goats’ ORS.
