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Detection of Expression Alteration of Cytokines in the Intestine of Balb/c Mice Infected with Cryptosporidium parvum using Relative Fluorescence Quantitative PCR Method

Detection of Expression Alteration of Cytokines in the Intestine of Balb/c Mice Infected with Cryptosporidium parvum using Relative Fluorescence Quantitative PCR Method

Liyun Chang1, Yingbin Chen1, Qian Kang1, JianhuaQin1,* and Zhiyong Liu2,*

1College of Veterinary Medicine, Agricultural University of Hebei, Baoding 071001, China
2Tangshan Animal Disease Prevention and Control Center, Thangshan, Hebei, 064001, China
 
Liyun Chang and Yingbin Chen contributed equally to this work.

* Corresponding author: qjhqqq@126.com; tslzy2005@163.com

Fig. 1.

The electrophoresis result of primers of real-time RT-qPCR. M: DL-1000Marker; 1:IL-2 2:IL-4 3:IL-6 4: TNF-α 5: GAPDH 6: IFN-γ.

Fig. 2.

The amplification curves of GAPDH, IFN-γ, TNF-α, IL-2, IL-4 and IL-6. 1-5: The dilution factor is 1×105-1×101 6: H2O.

Fig. 3.

The melting curves of GAPDH gene, IFN-γ, TNF-α, IL-2, IL-4 and IL-6.

Fig. 4.

Relative expression of mouse cytokine TNF-α. *means P< 0.05, **means P< 0.01, ***means P< 0.001.

Fig. 5.

Relative expression of mouse cytokine TNF-γ. *means P< 0.05, **means P< 0.01, ***means P< 0.001.

Fig. 6.

Relative expression of mouse cytokine IL-2. *means P< 0.05, **means P< 0.01, ***means P< 0.001.

Fig. 7.

Relative expression of mouse cytokine IL-4. *means P< 0.05, **means P< 0.01, ***means P< 0.001.

Fig. 8.

Relative expression of mouse cytokine IL-6. *means P< 0.05, **means P< 0.01, ***means P< 0.001.

Fig. 9.

Relative expression of mouse cytokine IL-4. *means P< 0.05, **means P< 0.01, ***means P< 0.001.

Fig. 10.

Relative expression of mouse cytokine IL-6. *means P< 0.05, **means P< 0.01, ***means P< 0.001.

Pakistan Journal of Zoology

December

Vol. 53, Iss. 6, Pages 2001-2521

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