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DNA Bar-Coding Based Identification of Neverita didyma (Roding 1798) and its Phylogeny

DNA Bar-Coding Based Identification of Neverita didyma (Roding 1798) and its Phylogeny

Amna Sulaman1, Qurat-ul-Ain Ijaz1, Muhammad Shafi2 and Faiz Muhammad1*

The Phylum Mollusca’s class Gastropoda contains a diverse range of organisms that live in several environments. Neverita didyma is a commercially important snail that is also known as the moon shell or bladder moon snail. Its molecular identification as N. didyma was confirmed in this investigation. The current study’s sequence has been submitted to NCBI under the accession number ON358159. It showed 91.36% similarity with the sequence under accession number NC046594. Neighbour- joining tree revealed that N.didyma from Pakistani coastal waters is distinctly clustered with other species of N. didyma. The present molecular-based confirmation of this species will be helpful for taxonomists, and biodiversity monitors.

 
*      Corresponding author: [email protected]

Fig. 1.

Photomorph of N. didyma collected at Keti Bandar during low tide on the Pakistan coast. The dorsal side of the N. didyma is shown in A, and the pod view is shown in B.

Fig. 2.
A neighbor joining tree depicting gastropod species evolutionary relationships. The species obtained from the Pakistani marine environment are shown in red circles.

Pakistan Journal of Zoology

October

Pakistan J. Zool., Vol. 56, Iss. 5, pp. 2001-2500

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