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Comparison of Two Set Pathotypic-Specific Primers to Detect Newcastle Disease Virus

Comparison of Two Set Pathotypic-Specific Primers to Detect Newcastle Disease Virus

Dwi Desmiyeni Putri1*, Nurhayati1, Intan Kamilia Habsari1, Ni Luh Putu Ika Mayasari2 

1Department of Animal Husbandry, Politeknik Negeri Lampung. Lampung, Indonesia; 2School of Veterinary Medicine and Biomedical Science IPB University, Bogor, Indonesia.

*Correspondence | Dwi Desmiyeni Putri, Department of Animal Husbandry, Politeknik Negeri Lampung. Lampung, Indonesia; Email: desmiyenidwi@gmail.com 

Figure 1

Complementary Nucleotide sequence between FA/FB, FA/FC primers and ND Isolates 

Figure 2

Complementary Nucleotide Sequence between Fout-S/Fout-AS primers and ND Isolates 

Figure 3

Fusion gene amplification results by FA/FB and FA/FC primers; M= Molecular size marker; 1, 2 = NDV/Ck/Bogor/011; 3, 4= NDV/Ck/GnSindur/014; 5, 6= NDV/Ck/Cianjur/015; 7, 8 = NDV/Ck/Bogor/015; 1, 3, 5, 7 = Amplification by FA/FB primer; 2, 4, 6, 8 = Amplification by FA/FB primer. 

Figure 4

Fusion gene amplification results by Fout-S/Fout-AS primers; M= Molecular size marker; 1= Negative Control (H2O), 2 = NDV/Ck/Bogor/011; 3= NDV/Ck/GnSindur/014; 4 = NDV/Ck/Cianjur/015; 5 = NDV/Ck/Bogor/015 

Advances in Animal and Veterinary Sciences

May

Vol. 12, Iss. 5, pp. 802-993

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