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Co-Treatment of Caffeic Acid Phenethyl Ester with Chitosan Nanoparticles Inhibits DNA Methylation in HepG2 Cells

Co-Treatment of Caffeic Acid Phenethyl Ester with Chitosan Nanoparticles Inhibits DNA Methylation in HepG2 Cells

Faisal Alzahani1* and Mohammed Abu El-Magd2*

1Department of Biochemistry, Faculty of Science, Embryonic Stem Cells Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, 21589, Saudi Arabia
2Department of Anatomy, Faculty of Veterinary Medicine, Kafrelsheikh University, Egypt.
 
*      Corresponding author: faahalzahani@kau.edu.sa, mohamed.abouelmagd@vet.kfs.edu.eg

Fig. 1.

Transmission electron microscope showed the presence of CNPs with various sizes ranging from 150 to 300 nm. Scale bar = 300 nm.

Fig. 2.

The cytotoxic potential of CAPE and CNPs on HepG2 cells. A representative graph displaying the IC50 value as revealed by the MTT assay. Cells were treated with CAPE or CNPs at serial concentrations from 3.125 to 100 μg/ml and were incubated for 24 h.

Fig. 3.

Effect of CAPE and/or CNPs on the expression of Bax (A) and Bcl2 (B) genes in HepG2 cells as detected by qPCR. Cells were treated with CAPE and CNPs alone or in combination (CAPE+CNPs) at doses of their IC50 and incubated for 24 h. Data were presented in the form of fold change mean ± SEM, n = 5/group. Different letters above means (as presented by columns plus error bars) refer to significant differences at P<0.05. All groups compared to each other.

Fig. 4.

Effect of CAPE and/or CNPs on the Global DNA methylation quantities in HepG2 cells. Cells were treated with CAPE and CNPs alone or in combination (CAPE+CNPs) at doses of their IC50 and incubated for 72 h. Data were presented in the form of % mean ± SEM, n = 5/group. Different letters above means (as presented by columns plus error bars) refer to significant differences at P<0.05. All groups compared to each other. The control group was assigned a value of 100%.

Fig. 5.

Effect of CAPE and/or CNPs on the expression of DNA methylation-related genes DNMT1 (A) and Ube2e2 (B) in HepG2 cells as detected by qPCR. Cells were treated with CAPE and CNPs alone or in combination (CAPE+CNPs) at doses of their IC50 and incubated for 24 h. Data were presented in the form of fold change mean ± SEM, n = 5/group. Different letters above means (as presented by columns plus error bars) refer to significant differences at P<0.05. All groups were compared to each other.

Pakistan Journal of Zoology

February

Pakistan J. Zool., Vol. 56, Iss. 1, pp. 01-501

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