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Autologous Platelets-Rich Plasma Gel Promotes Neo-Vascularization and Re-Epithelization in Cutaneous Wound Healing in Rescued Horses

Autologous Platelets-Rich Plasma Gel Promotes Neo-Vascularization and Re-Epithelization in Cutaneous Wound Healing in Rescued Horses

Muhammad Talha Sajjad1, Hamid Akbar1*, Muhammad Arif Khan1, Muhammad Hassan Mushtaq3, Shehla Gul Bokhari1, Muhammad Abid Hayat4 and Ghulam Mustafa2

1University of Veterinary and Animal Sciences, Faculty of Veterinary Sciences, Department of Veterinary Surgery and Pet Sciences, 54000 Lahore, Pakistan
2University of Veterinary and Animal Sciences, Faculty of Veterinary Sciences, Department of Pathology, 54000 Lahore, Pakistan 
3University of Veterinary and Animal Sciences, Faculty of Veterinary Sciences, Department of Epidemiology and Public Health, 54000, Lahore, Pakistan 
4Northeast Agricultural University, College of Veterinary Medicine, Department of Veterinary Surgery, 150030 Harbin, P.R. China
 
*      Corresponding author: hamid.akbar@uvas.edu.pk

Fig. 1.

Ultrasonographical evaluation of neo-vascularization in the cutaneous wound of both groups. A; PRP treated wound obtained at day 14 showed mild development of blood vessels. B; Control wound showed no development of neo-vascularization at day 14. C; PRP treated wound at day 28 showed increased development of neo-vascularization at day 28. D; Control wound at day 28 showed mild development of neo-vascularization.

Fig. 2.
Ultrasonographic scoring of neovascularization between the both groups. A, indicates level of neovascularization in PRP and Control wounds at day 14. B, indicates neovascularization in both groups. ‘**’ indicated the differences were significant in the PRP-treated group compared with the control group at day 28 (P<0.01).
Fig. 3.

Histopathological evaluation of neo-vascularization in the cutaneous wound of both groups. Bar=50um A, PRP treated wound obtained at day 14 showed mild development of blood vessels. B, Control wound showed no development of neo-vascularization at day 14. C, PRP treated wound at day 28 showed increased development of neo-vascularization at day 28. D, Control wound at day 28 showed mild development of neo-vascularization.

Fig. 4.

Histopathological scoring of neo-vascularization and fibroblast between the both groups. A, indicates level of neovascularization in PRP and Control wounds at day 14. B, indicates neovascularization in both groups at 28 days. C, indicates level of number of fibroblasts in PRP and Control wounds at day 14. D, indicates level of number of fibroblasts in both groups at 28 days. **, indicated the differences were significant in the PRP-treated group compared with the control group at day 28 (P<0.01).

Fig. 5.

Cytokeratin staining in tissues obtained at Days 14 and 28 after wounding for Re-epithelization in both groups. A, PRP gel treated wound tissue displays accelerated epithelial growth, increased proliferation of connective tissue at day 14. B, Control wound tissue displays no development of epithelial growth, no basal lamina growing, and mild proliferation of connective tissue at day 14. C, PRP gel-treated wound tissue displays increased epidermal growth, intact basal layer, multiple layers of stratum spinosum visible, stratum corneum also visible, and increased fibrous connective tissue on day 28. D, Control wound tissue mild epithelial growth, basal lamina growing, and mild proliferation of connective tissue at 28 days.

Fig. 6.

Histopathological scoring of re-epithelization between the both groups. A, indicates level of epithelial differentiation in PRP and Control wounds at day 14. B, indicates level of epithelial differentiation in both groups. ‘**’  indicates the differences were significant in the PRP-treated group compared with the control group at day 28 (P<0.01).

Pakistan Journal of Zoology

February

Pakistan J. Zool., Vol. 56, Iss. 1, pp. 01-501

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