Addition of L-Cysteine and Vitamin E to Semen Diluent Enhances Freeze-thawed Spermatozoa Characteristics in Crossbred Cattle Bulls under Subtropical Environment
Addition of L-Cysteine and Vitamin E to Semen Diluent Enhances Freeze-thawed Spermatozoa Characteristics in Crossbred Cattle Bulls under Subtropical Environment
Ibrar Muhammad Khan1, Dezhi Xu1, Zubing Cao1, Hongyu Liu1, Adnan Khan2, Sajid Ur Rahman3, Jam Zaheer Ahmed4, Muhammad Akmal Raheem5 and Yunhai Zhang1*
The effect of L-cysteine and vitamin E supplemented diluents on freeze-thawed spermatozoa traits in Achai × Jersey bulls (Mean±SEM). Control diluent containing no additives; L-c-1 diluent containing 5 mmol of L-cysteine; L-c-2 diluent containing 7.5 mmol of L-cysteine; L-c-3 containing 10 mmol of L-cysteine; Vit-E-1 diluent containing 1.2 mmol of vitamin E; Vit-E-2 diluent containing 2.4 mmol of vitamin E; Vit-E-3 diluent containing 4.8 mmol of vitamin E; TM, total motility; PM, progressive motility; MF, membrane functionality; DNAI, DNA integrity; different letters show significant difference (P<0.05).
The impact of L-cysteine and vitamin E supplemented diluents on freeze-thawed spermatozoa parameters in Achai × Jersey bulls (Mean±SEM). Control diluent containing no additives; L-c-1 diluent containing 5 mmol of L-cysteine; L-c-2 diluent containing 7.5 mmol of L-cysteine; L-c-3 containing 10 mmol of L-cysteine; Vit-E-1 containing 1.2 mmol of vitamin E; Vit-E-2 containing 2.4 mmol of vitamin E; Vit-E-3 containing 4.8 mmol of vitamin E. AI, acrosomal integrity; MMP, mitochondria membrane potential; SV, sperm viability; different letters show significant difference (P<0.05).
