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Addition of L-Cysteine and Vitamin E to Semen Diluent Enhances Freeze-thawed Spermatozoa Characteristics in Crossbred Cattle Bulls under Subtropical Environment

Addition of L-Cysteine and Vitamin E to Semen Diluent Enhances Freeze-thawed Spermatozoa Characteristics in Crossbred Cattle Bulls under Subtropical Environment

Ibrar Muhammad Khan1, Dezhi Xu1, Zubing Cao1, Hongyu Liu1, Adnan Khan2,  Sajid Ur Rahman3, Jam Zaheer Ahmed4, Muhammad Akmal Raheem5 and Yunhai Zhang1*

1Anhui Provincial Laboratory of Local Livestock and Poultry Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui, China, 230036
2Department of Animal Genetics, Breeding and Reproduction College of Animal Science and Technology, China Agricultural University, Beijing, China
3Key Laboratory of Animal Parasitology of Ministry of Agriculture, Laboratory of Quality and Safety Risk Assessment for Animal Products on Biohazards (Shanghai) of Ministry of Agricultural Sciences, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China  
4State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning, Guangxi, China 
5Key Laboratory of Veterinary Pathobiology and Disease Control, College of Animal Science and Technology, Anhui Agricultural University, Hefei, China

*      Corresponding author: yunhaizhang@ahau.edu.cn

Fig. 1.

The effect of L-cysteine and vitamin E supplemented diluents on freeze-thawed spermatozoa traits in Achai × Jersey bulls (Mean±SEM). Control diluent containing no additives; L-c-1 diluent containing 5 mmol of L-cysteine; L-c-2 diluent containing 7.5 mmol of L-cysteine; L-c-3 containing 10 mmol of L-cysteine; Vit-E-1 diluent containing 1.2 mmol of vitamin E; Vit-E-2 diluent containing 2.4 mmol of vitamin E; Vit-E-3 diluent containing 4.8 mmol of vitamin E; TM, total motility; PM, progressive motility; MF, membrane functionality; DNAI, DNA integrity; different letters show significant difference (P<0.05).

Fig. 2.

The impact of L-cysteine and vitamin E supplemented diluents on freeze-thawed spermatozoa parameters in Achai × Jersey bulls (Mean±SEM). Control diluent containing no additives; L-c-1 diluent containing 5 mmol of L-cysteine; L-c-2 diluent containing 7.5 mmol of L-cysteine; L-c-3 containing 10 mmol of L-cysteine; Vit-E-1 containing 1.2 mmol of vitamin E; Vit-E-2 containing 2.4 mmol of vitamin E; Vit-E-3 containing 4.8 mmol of vitamin E. AI, acrosomal integrity; MMP, mitochondria membrane potential; SV, sperm viability; different letters show significant difference (P<0.05).

Pakistan Journal of Zoology

August

Vol. 53, Iss. 4, Pages 1201-1601

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