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Acetylcholinesterase Inhibitory Activity of Venom of Hottentota tamulus (Fabricius) and Androctonus finitimus (Pocock) (Scorpiones: Buthidae) in Musca domestica

Acetylcholinesterase Inhibitory Activity of Venom of Hottentota tamulus (Fabricius) and Androctonus finitimus (Pocock) (Scorpiones: Buthidae) in Musca domestica

Naila Riaz1, Hafiz Muhammad Tahir2, Muhammad Khalid Mukhtar1, Ali Hassan2, Shaukat Ali2, Shafaat Yar Khan1

1Department of Zoology, University of Sargodha, Sargodha, Pakistan
2Department of Zoology, Government College University, Lahore, Pakistan

Corresponding author: Hafiz Muhammad Tahir, hafiztahirpk1@yahoo.com

 

Figure 1

Scorpion species used in the study. A: Androctonus finitimus and B: Hottentota tamulus.

Figure 2:

Peptide fractions of scorpion species (A= Hottentota tamulus and B= Androctonus finitimus) were separated by HPLC.

Figure 3:

Comparison of mean percentage mortality rate in M. domestica adults caused by specific doses of selected scorpion species venom peptides and Chlorpyrifos. *(-ve control= Water, +ve control= Chlorpyrifos, Ht1= 10µg/ml of H. tamulus venom peptide, Ht2= 20µg/ml of H. tamulus venom peptide, Ht3= 30µg/ml of H. tamulus venom peptide, Af1= 10µg/ml of A. finitimus venom peptide, Af2= 20µg/ml of A. finitimus venom peptide and Af3= 30µg/ml of A. finitimus venom peptide). * Data represented as Mean± SEM.

Figure 4

Comparison of AChE activity (Optical density) in M. domestica adults caused by specific doses of selected scorpion species venom peptides and Chlorpyrifos. (-ve control= Water, +ve control= Chlorpyrifos, Ht1= 10µg/ml of H. tamulus venom peptide, Ht2= 20µg/ml of H. tamulus venom peptide, Ht3= 30µg/ml of H. tamulus venom peptide, Af1= 10µg/ml of A. finitimus venom peptide, Af2= 20µg/ml of A. finitimus venom peptide and Af3= 30µg/ml of A. finitimus venom peptide). Different alphabets indicates the significant difference between the different groups, same alphabet indicates non-significant difference in the groups. Optical density was recorded with absorbance at wavelength of 412nm

Punjab University Journal of Zoology

December

Vol. 35, Iss. 2, pp. 167-268

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