Present study was conducted to report the effect of 1mg / ml of solvent / Kg body weight GABAB receptor antagonist (CGP55845; 3-N [1-(S)-(3,4- dichlorophenyl) ethyl] amino-2-(S)- hydroxypropyl-P-benzyl-phosphinic acid), intraperitoneally injected for 12 days, on hematological and serum biochemical profile of 6 week old male albino mice. Blood samples from male albino mice (CGP55845 treated N = 14 and saline treated N = 16) were collected directly by cardiac puncture and used for estimation of hematological and some serum biochemical parameters. The hematological parameters did not show any significant variation between the two groups. From amongst serum biochemical parameters triglycerides (P = 0.02) in CGP55845 treated mice showed significant increase compared to the control mice. All other studied parameters remained unaffected indicating that CGP55845 can be injected intraperitonially for the treatment of neurological ailments.
Short Communication · Volume 50, Issue 1, 19 January 2018 · Open Access
Effect of GABAB Receptor Antagonist (CGP55845) on the Hematological and Serum Biochemical Profile of Adult Male Albino Mice
· ·
Affiliations & Notes ⌄
- 1Institute of Pure and Applied Biology, Zoology Division, Bahauddin Zakariya University, Multan 60800, Pakistan , Rabia Qurashi and Qurat ul Ane Gillani contributed equally to the manuscript. , * Corresponding authors: anneegillani@gmail.com
- 2furhan.iqbal@bzu.edu.pk
Article Info ⌄
- Journal:
- Pakistan Journal of Zoology
- Published:
- 19 January 2018
From the article
- All the parameters studied did not show any significant difference between the two groups, except for triglycerides which was significantly higher in treated group.
- This correlation was also observed during present studies as triglyceride level was significantly higher and HDL value was lower in CGP55845 treated mice indicating a normal blood chemistry and physiology following CGP 55845 application (Table I).
Selected from the article's own text.
Abstract
Materials and methods
Adult male (6 week old) albino mice (n = 30; 16 control and 14 treated with CGP55845), obtained from Zoology Department, Punjab University, Lahore, were housed in individual cages (22±1°C) during experiment and provided with standard mouse diet and water. Ethical committee of Zoology Department (Bahaudin Zakarya University Multan) had approved this experimental protocols and animal handling procedure.
Treated group received dose of GABAB receptor antagonist CGP55845 (1mg/kg body weight) for 12 days by injecting intraperitoneally while the control mice received saline solution for 12 days in parallel. The blood was obtained through retro orbital sinus or by cardiac puncture of mice and used for estimation of hematological parameters such as total white (TWBC) and red blood cells (TRBC), blood glucose level, packed cell volume (PCV) and mean corpuscular volume (MCV) and serum biochemical parameters such as alanine transaminase (ALT), aspartate transaminase (AST), total protein, cholesterol, high density lipoprotein (HDL), low density lipoprotein (LDL) and triglycerides using Hitachi 902 automatic analyzer (Japan) following Khadim et al. (2014).
To analyze the data, statistical package Minitab (version 16, Pennsylvania) was used. Data was expressed in Mean ± standard deviation. Comparison of various hematological and studied serum biochemical parameters between two treatments was carried out by applying two sample t-test.
Table I.- Comparison of various hematological and biochemical parameters between GABAB receptor antagonist (CGP55845) and saline treated (control) male albino mice. Data is expressed as Mean ± Standard deviation. Ranges of values are given below the Means. P-value indicates the results of 2 sample t-test.
| Parameters | Control (n = 16) (Range) | CGP55845 treated (n = 14) (Range) |
| Hematological profile | ||
| Glucose (mg/dl) | 209.7 ± 17.8 (74 – 293) | 185.2 ± 3.72 (118 -218) |
| PVC (%) | 41.5 ± 3.3 (28 – 56) | 39.22 ± 0.91 (30 – 52) |
| TRBC (x103) | 24.48 ± 0.64 (12.1 - 34) | 21.9 ± 0.97 (13.1 – 40.5) |
| TWBC (x103) | 10.72 ± 0.25 (6 – 15.5) | 10.43 ± 0.20 (7.75 – 13.4) |
| MCV (fp) | 1.85 ± 0.26 (1.06 – 4.13) | 2.10 ± 0.11 (0.81 – 3.90) |
| Serum biochemical profile | ||
| Total Protein (g/dl) | 5.65 ± 0.16 (2.07 – 9.03) | 6.40 ± 0.18 (3.36 – 9.47) |
| Cholesterol (mg/dl) | 162.4 ± 4.6 (57 – 292) | 136.2 ± 4.36 (64 – 258) |
| Triglycerides (mg/dl) | 191 ± 8.4 (73 – 381) | 335 ± 14* (78 – 644) |
| HDL (mg/dl) | 39.1 ± 1.35 (25 – 61) | 35 ± 1.85 (13 – 63) |
| LDL (mg/dl) | 92.2 ± 4.31 (32 – 188) | 52.6 ± 5.53 (7.2 – 169) |
| ASAT (U/l) | 198 ± 18 (0.2 – 270) | 180 ± 14 (0.1 – 393) |
| ALAT (U/l) | 265 ± 16 (61 – 508) | 182 ± 20 (31 – 342) |
P > 0.05, non-significant; *P < 0.05, least significant. PCV, packed cell volume; TRBC, total red blood cell count; TWBC, total white blood cell count; MCV, mean corpuscular volume; HDL, high density lipoprotein; LDL, low density lipoprotein; ASAT, aspartate aminotransferase; ALAT, alanine aminotransferase.
Results and discussion
Table I shows the various hematological and serum biochemical parameters of CGP55845 treated and untreated albino mice. All the parameters studied did not show any significant difference between the two groups, except for triglycerides which was significantly higher in treated group.
GABA is a four carbon non-protein amino acid which acts as a major inhibitory neurotransmitter in the central nervous system and it play controlling role in balancing the excitability and inhibitory state in cortex and hippocampus . Our results are in agreement with a recent study conducted by Aslam et al. (2015) who showed that CGP 55845 can be supplemented in both male and female albino mice without affecting the cell counts at any stage of their adult life.
Butyrate is known for its weight loss effects by effecting triglycerides and lipoproteins is the conjugate base of butyric acid (Masud et al., 2016). It has been reported that higher concentrations of triglyceride in serum leads to lower high density lipoproteins (HDL) concentrations . This correlation was also observed during present studies as triglyceride level was significantly higher and HDL value was lower in CGP55845 treated mice indicating a normal blood chemistry and physiology following CGP 55845 application (Table I).
Conclusion
Our results indicated that GABAB receptor antagonist, CGP55845, has no effect on the studied hematological and most of the serum biochemical parameters of male albino mice as all these parameters had non-significant (P > 0.05) variations when compared between saline and CGP55845 treated male albino mice, indicating normal blood chemistry and physiology. So it is recommended that CGP 55845 may be administrated intravenously in subjects for the treatment of neurological ailments, where required.
Acknowledgement
The authors are grateful to Higher Education Commission (HEC) of Pakistan for providing research grant for this study under Indigenous PhD Scholarship Scheme.
Statement of conflict of interest
Authors have declared no conflict of interest.
References
- Aslam, S., Gillani, Q. and Iqbal, F., 2015. Pakistan J. Zool., 47: 1601-1604.
- Bowery, N.G., 2006. Curr. Opin. Pharmacol., 6: 37-43. https://doi.org/10.1016/j.coph.2005.10.002[CrossRef]
- Gillani, Q., Akbar, A., Ali, M. and Iqbal, F., 2015. Neurol. Sci., 36: 961-969. https://doi.org/10.1007/s10072-015-2205-2[CrossRef]
- Hernandez, C.M., Gearhart, D.A., Parikh, V., Hohnadel, E.J., Davis, L.W., Middlemore, M.L., Warsi, S.P., Waller, J.L. and Terry, A.V., 2006. Exp. Therap., 316: 679-694. https://doi.org/10.1124/jpet.105.093047[CrossRef]
- Huang, J., Mei, L. and Wu, H., 2007. World J. Microbiol. Biotechnol., 23: 865-871. https://doi.org/10.1007/s11274-007-9357-z[CrossRef]
- Iqbal, F. and Gillani, Q.A.U., 2016. In: GABAB receptors (ed., G. Colombo). Springer International Publishing, AG, Cham.
- Khadim, N., Iqbal, S., Gillani, Q.A. and Iqbal, F., 2014. J. Anim. Pl. Sci., 24: 374-379.
- Luscher, B., Shen, Q. and Sahir, N., 2011. Mol. Psychiat., 16: 383-406. https://doi.org/10.1038/mp.2010.120[CrossRef]
- Masud, F., Shakoori, T.A., Khawaja, K.I., Ali, M. and Ameer, F., 2016. Pakistan J. Zool., 48: 1343-1348.
- Mayse, J.D., 2009. Turning back the clock: CGP55845 reversed mild cognitive impairment in Fischer 344 rats. Texas A and M University, USA.
- Paulsen, O. and Moser, E.I., 1998. Trend Neurosci., 21: 273-278. https://doi.org/10.1016/S0166-2236(97)01205-8[CrossRef]
Services
Article usage statistics
Loading…