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Prevalence and Genotypic Analysis of Hepatitis C Virus Isolated from Liver Center, Faisalabad, Pakistan

Prevalence and Genotypic Analysis of Hepatitis C Virus Isolated from Liver Center, Faisalabad, Pakistan

Muhammad Siddique1, Muhammad Shafique2,*,Abdul Mateen3, Naveed Shahzad4, Bilal Aslam2, Nasir Ahmad1 and Muhammad Usman Qamar2

1Health Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad
2Department of Microbiology, Government College University, Faisalabad, 
3Department of Zoology, Wildlife and Fisheries, University of Agriculture, Faisalabad 
4School of Biological Sciences, University of the Punjab, Lahore

*      Corresponding author: drmshafique@gcuf.edu.pk; shafique.nl@gmail.com

 

Fig. 1.

the HCV-RNA positive samples. M-50bp ladder. NC, negative control; PC, positive control; T, tested samples.

Fig. 2.

HCV genotyping by RFLP. M-50bp ladder, T1-T4: tested samples digested with HaeIII and RsaI, T5-T8: tested samples digested with ScrfI and HinfI and T9-T12: tested samples digested with MvaI and HinfI.

Fig. 3.

HCV genotyping by RFLP. M-50bp ladder, T1, T5, T9: showing genotypes 1. T2, T3, T4, T6, T7, T8, T10, T11 and T12: tested samples showing genotype 3.

Fig. 4.

Multiplex PCR with Mix-A of RT-PCR positive samples. M-50bp ladder, T1-T15: tested samples.

Fig. 5.

Multiplex PCR with Mix-B of RT-PCR positive samples. M-50bp ladder; T4, T5, T7, T9, 12, 13 and 14: Amplified products with band size 232bp.

Pakistan Journal of Zoology

December

Vol. 51, Iss. 6, Pages 1999-2399

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