Prevalence and Genotypic Analysis of Hepatitis C Virus Isolated from Liver Center, Faisalabad, Pakistan
Prevalence and Genotypic Analysis of Hepatitis C Virus Isolated from Liver Center, Faisalabad, Pakistan
Muhammad Siddique1, Muhammad Shafique2,*,Abdul Mateen3, Naveed Shahzad4, Bilal Aslam2, Nasir Ahmad1 and Muhammad Usman Qamar2
Fig. 1.
the HCV-RNA positive samples. M-50bp ladder. NC, negative control; PC, positive control; T, tested samples.
Fig. 2.
HCV genotyping by RFLP. M-50bp ladder, T1-T4: tested samples digested with HaeIII and RsaI, T5-T8: tested samples digested with ScrfI and HinfI and T9-T12: tested samples digested with MvaI and HinfI.
Fig. 3.
HCV genotyping by RFLP. M-50bp ladder, T1, T5, T9: showing genotypes 1. T2, T3, T4, T6, T7, T8, T10, T11 and T12: tested samples showing genotype 3.
Fig. 4.
Multiplex PCR with Mix-A of RT-PCR positive samples. M-50bp ladder, T1-T15: tested samples.
Fig. 5.
Multiplex PCR with Mix-B of RT-PCR positive samples. M-50bp ladder; T4, T5, T7, T9, 12, 13 and 14: Amplified products with band size 232bp.
February 2020
Vol. 52, Iss. 1, Pages 1-424
