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Identification and Isolation of the Specific Allergens from Mutton by Western Blot, Chromatography and MALDI-TOF/TOF-MS

Identification and Isolation of the Specific Allergens from Mutton by Western Blot, Chromatography and MALDI-TOF/TOF-MS

Jia Chen*, Yuanxi Deng and Hui Xu

Department of Food and Bioengineering, Bengbu College, Bengbu, 233030, China

*      Corresponding author: bojourjia@163.com

 

Fig. 1.

The SDS-PAGE profile of crude protein extracts from mutton. M, marker; 1, protein extracts from mutton.

Fig. 2.

The western blot profile of protein extracts with sera from rats. M, marker; 1, positive sera; 2, negative sera.

Fig. 3.

Ion-exchange chromatography (DEAE-52 cellulose) of crude protein extract from mutton with a linear NaCI gradient and divided into four fractions.

Fig. 4.

Purification of peak 2 on Gel filtration chromatography Sephadex G-75 (Flow-rate, 1.0 mL/min; fraction volume, 10 mL).

Pakistan Journal of Zoology

October

Vol. 50, Iss. 5, Pages 1601-1998

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