Identification and Isolation of the Specific Allergens from Mutton by Western Blot, Chromatography and MALDI-TOF/TOF-MS
Identification and Isolation of the Specific Allergens from Mutton by Western Blot, Chromatography and MALDI-TOF/TOF-MS
Jia Chen*, Yuanxi Deng and Hui Xu
Fig. 1.
The SDS-PAGE profile of crude protein extracts from mutton. M, marker; 1, protein extracts from mutton.
Fig. 2.
The western blot profile of protein extracts with sera from rats. M, marker; 1, positive sera; 2, negative sera.
Fig. 3.
Ion-exchange chromatography (DEAE-52 cellulose) of crude protein extract from mutton with a linear NaCI gradient and divided into four fractions.
Fig. 4.
Purification of peak 2 on Gel filtration chromatography Sephadex G-75 (Flow-rate, 1.0 mL/min; fraction volume, 10 mL).
October 2018
Vol. 50, Iss. 5, Pages 1601-1998
