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Identification and Isolation of the Specific Allergens from Mutton by Western Blot, Chromatography and MALDI-TOF/TOF-MS

Identification and Isolation of the Specific Allergens from Mutton by Western Blot, Chromatography and MALDI-TOF/TOF-MS

Jia Chen*, Yuanxi Deng and Hui Xu

Department of Food and Bioengineering, Bengbu College, Bengbu, 233030, China

*      Corresponding author: bojourjia@163.com

 

ABSTRACT

To study the specific allergen in mutton, soluble protein from mutton was extracted, and separated by SDS-PAGE. With the positive and negative sera of rats, the special allergen was identified with the Western Blot, showing that the 12kDa was the specific allergen. The protein of 12kDa was then analyzed by ion exchange and gel filtration chromatography, and the MALDI-TOF/TOF-MS search on the Internet. The results indicate that the protein was diamine- N-acetyltransferase.
 

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Pakistan Journal of Zoology

August

Vol. 50, Iss. 4, Pages 1199-1600

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