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Expression Analysis of Oxidative Stress Induced Genes in Liver and Heart Tissues in Response to Doxorubicin

Expression Analysis of Oxidative Stress Induced Genes in Liver and Heart Tissues in Response to Doxorubicin

Uzma Jabeen1, Irfan Khan2, Nadia Naeem2, Asmat Salim2,* and Waseem Ahmed1

1Department of Biochemistry, and Department of Biotechnology Federal Urdu University of Arts Science and Technology, Gulshan-e-Iqbal Campus, Karachi-75300
2Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, Karachi-75270

*      Corresponding author: asmat.salim@iccs.edu

 

ABSTRACT

Drugs used in chemotherapy that target rapidly dividing cells, also exert toxic effects on healthy tissues. Doxorubicin (DOX) is a commonly used chemotherapeutic drug. Oxidative stress plays an important role in DOX-induced toxicity. This study was aimed at analyzing the expression levels of genes that are induced in response to oxidative stress in heart and liver tissues following DOX administration to rats. In this study, DOX was administered to rats intraperitoneally (i. p.) at 3 mg/kg at alternate days for two weeks. Heart and liver tissues were harvested and the mRNA levels of NAD(P)H quinone dehydrogenase 1 (Nqo1), glutathione peroxidase (Gpx1) and isocitrate dehydrogenase-1 (Idh1) were analyzed by RT-PCR. We observedvariable pattern of gene expression was observed. Nqo1 and Idh1 genes were upregulated significantly in heart but not in liver tissue. Gpx1 seems to be unaffected both in heart and liver tissues. It is concluded from this study that toxicity due to doxorubicin is variable in terms of expression of certain oxidative stress induced genes and is tissue dependent. These genes therefore can be a potential target for future treatment of cardiotoxicity induced by doxorubicin.
 

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Pakistan Journal of Zoology

December

Vol. 51, Iss. 6, Pages 1999-2399

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