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Cloning, Polymorphism and Tissue Expression of TAC3 Gene in Sheep

Cloning, Polymorphism and Tissue Expression of TAC3 Gene in Sheep

Wenping Hu1, Xiangyu Wang1, Xiaodi Sun2, Ran Di1, Qiuyue Liu1, Zhangyuan Pan1, Xiaofei Guo1, Xiaohan Cao1, Jinyu Wang2, Yingjie Zhang3, Mei Jin4, Yuze Yang5 and Mingxing Chu1*

1Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R. China.
2College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, P.R. China.
3College of Animal Science and Technology, Hebei Agricultural University, Baoding 071000, P.R. China.
4College of Life Science, Liaoning Normal University, Dalian 116029, P.R. China.
5Beijing General Station of Animal Husbandry, Beijing, 100101, P.R. China.
 
Wenping Hu and Xiangyu Wang contributed equally.

*  Corresponding author: mxchu@263.net

Fig.

(A) PCR products of P1 - P5 primers. (B) Nucleotide mutations at locus 327 and locus 1803 in sheep TAC3 gene. (C) Electrophoresis analysis of RFLP products of primer P1 and P3. P1: Bands 1, 2, 3 and 4 were CC genotype; Bands 5 and 6 were CT genotype; Band 7 was TT genotype; M: DNA Marker 2000. P3: Bands 1 and 6 were AA genotype; Bands 2, 3, 5,7 and 8 were GG genotype; Bands 4 and 9 were AG genotype; M: DNA Marker 2000.

Pakistan Journal of Zoology

October

Vol. 52, Iss. 5, Pages 1631-2026

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