Alexander Tsybulsky1*, Tatyana Tabakaeva1, Anna Klimovich2, Michail Shchelkanov1, Eduard Kostetsky1, Maksim Aliev1, Anton Degtyarenko3
...6) Expression of p53 and gadd45g genes in WBC. The concentration of the viral p27-FeLV antigen in the blood plasma was determined by ELISA. The total DNA and total RNA was extracted from the WBC. The cDNA was obtained using an mRNA matrix. Qualitative and quantitative analyses of FeLV in WBC as well as expression of genes of interest were conducted using real-time PCR (qPCR). Results. Cats with initial signs of leukaemic process activation in WBC showed the fo...
Alexander Tsybulsky1*, Eduard Kostetsky1, Anton Degtyarenko2, Michail Shchelkanov1,2,3
...;1, p53, rb1, cdkn2a and gadd45g genes in the BC and FCM tumor tissues, as well in the uterus tissue and WBCs using qPCR. Results. In cats with MG tumours, signs of lymphopenia and thrombocytopenia were found, these being especially pronounced in animals with BC. In cats with BC and FCM, there were significant differences in the expression patterns of the spectrum of target IFN system genes and a number of key cell cycle control factors. These differences were...
