Taghreed A. Hafiz1, Murad A. Mubaraki1*, Mohamed A. Dkhil2,3 and Saleh Al-Quraishy2
... were determined using a spectrophotometric method. The antiradical scavenging property and the maximum nontoxic concentration of the extract was determined using DPPH assay and MTT method, respectively. Serial concentrations of the extract not exceed the maximum nontoxic concentration were used against Vero cells for anti-HSV-1 and ant-HSV-2 effects by plaque assay. The results of the phytochemical analysis showed that the extract has high polyphenolic c...
Rimsha Nawaz1, Muhammad Javed1, Sidra Abbas1* and Safina Kousar2
...aiacol to tetraguaiacol, spectrophotometrically, at a wavelength of 470 nm. The peroxidase activity was measured in the selected organs exposed to various sub-lethal concentrations of iron chloride. The results revealed that peroxidase activity was increased significantly in both kidney and liver after exposure to iron chloride in all the treatments as compared to the control group. It was found that enzyme peroxidase had activity of 0.364±0.004 and 0.5...
Alim-un-Nisa1*, Sajila Hina1, Sania Mazhar1, Imran Kalim1, Ijaz Ahmad1, Naseem Zahra1, Shahid Masood1, Muhammad Khalid Saeed1, Qurat-ul-Ain Syed1 and Maida Asif2
... the attractiveness. The spectrophotometric analysis at 474nm of extracted color both in crude form and in candies was found to be stable at 4oC and showed decay in mean lutein concentration at higher temperatures i.e. 25oC and 45oC. The microbiological study of the extracted color and candies prepared in laboratory and dyed with T. erecta extract color showed that the color extracted from T.erecta itself do not p...
Sana Rana1, Sajid Abdullah1, Huma Naz2* and Khalid Abbas1
...AT activity was measured spectrophotometrically at A240 nm. Results showed that highest CAT activity was noted from the crude extract of control fish gills (140.66±0.71UmL-1) as compared to DM+END exposed fish (99.23±0.71UmL-1). After desalted the CAT activity in gills of both control and insecticides exposed fish was calculated as 73.45±0.71 and 42.77±1.36UmL-1, respectively. The highest specific activity was observed in crude CAT ...
Umar Farooq Gohar1, Hamid Mukhtar1,*, Ali Nawaz1, Ikram-ul-Haq1 and Asad Mehmmood2
...n was estimated by using spectrophotometric technique.
...
Rıfat Ertekin1*, Mete Ozkurt1, Gokhan Kus2 and Selda Kabadere1
...time PCR techniques. MTT spectrophotometric tests showed no significant effects on the survival rate of T-98G cells in 24 and 48 h. Therefore, T-98G cells were not analysed by flow cytometry and RT-PCR. When LNCaP cells were exposed to 1, 5, 10, 25, 50 and 100 μM doses of TA for 24 h, the rates of living cells were determined as 91%, 83%, 82%, 76%, 61% and 49%, respectively. When the same doses were applied for 48 h, the living cell rates were 90%, 83%, 82%...
Hafiz Muhammad Arsalan1, Maria Altaf1, Zeemal Seemab Amin1, Muhammad Khalil Ahmad Khan2, Anum Shahzadi1, Hina Mudasser1, Iqra Maqsood1, Nazia Gulshan1, Saira Naseem3
...nce was measured through spectrophotometric procedure. Serum MDA level jump high in patients (14.19) as compared to normal subjects (3.26). Serum Glutathione declined in disease persons (0.56) from healthy subjects (6.39). Serum Micronutrients level was also declined in RA patients as compared to healthy persons and substantial statistically (P=0.000). Serum Nitric Oxide jump high in patients (32.11) than normal persons (15.2). Abnormal concentration of reacti...
Zahid Hassan Tarar1, Muhammad Salik Ali Khan2*, Shahzada Munawar Mehdi3, Raza Salim4, Irfan Ahmad Saleem1, Saima Nazar5, Munir David2, Tahir Majeed6, Muhammad Sufyan Mughal7, Muhammad Saleem8, Umer Iqbal9, Muhammad Mazhar Iqbal10 and Muhammad Khalid Shaheen3
...Boron (B) was determined spectrophotometrically. Additionally, soil samples were also analysed for electrical conductivity (EC), soil pH and soil organic matter (SOM) contents. These characteristics were correlated with plant available micronutrients. The DTPA-extractable Zn, Cu, Fe, and Mn ranged from 0.07-5.60, 0.11-1.22, 0.74-7.45 and 0.02-3.39 mg/Kg soil, respectively. Boron ranged from 0.06-0.69 mg/Kg soil. Considering the critical limits set by National ...
Ayesha Noreen1, Amina Elahi1, Dilara Abbas Bukhari2 and Abdul Rehman1*
...ated by both Safranine O spectrophotometric and molybdene blue methods) at pH 7 and 37°C in the presence of Zn2+ as cofactor. The protein profile of B. cereus 3.1S, showed two bands of approximately 14 and 70 kDa, which had their possible role in arsenite oxidation. This was confirmed by transforming E. coli DH5α with plasmid DNA of B. cereus 3.1S. This arsenite resistant bacterial strain oxidized 76 and 86.5% As3...
Allam A. Megahed1; Hoda M.A. Waziri2; Khaled A. El-Dougdoug3; Badawi A. Othman3; Sirag M. Lashin1; Mohamed D. Hassanin1 and Mahmoud A. Ibrahim4
...paration estimated using spectrophotometric measurements at 260 nm was 3.004 mg/ml. The min, max nm, Amax./Amin., A260/A280 and A280/A260 ratios were 244, 260, 1.276, 1.512 and 0.662, respectively for BtMV. The corresponding figures of the purified virus preparation of Beet necrotic yellow vein virus (BNYVV) were 2.196 mg/ml, and the min, max nm, Amax./Amin., A260/A280 and A280/A260 were 248, 264, 1.606, 1.237, and 0.785, respectively. The polyclonal antibodie...
Amel, S.M. Abo-Senna1; M.A. Nasr-Eldin2; B.A. Othman3 and A.A. Megahed4
...days. The characteristic spectrophotometric data of the highly purified virus showing that maximum and minimum absorbance was 250 and 260 nm respectively; the ratio for A280/A260 was 1.004; and the Amax./Amin. was 1.099 with the final yield as 0.883 mg/ml. Electron microscopy showed the filamentous flexuous particles with about 750 X 12-15 nm. The virus was identified as ZYMV by RT-PCR technique using high specific primers as 113 bp fragment within the nuclear...
Semra Okur Gumusova1, Gul Fatma Yarim2, Cuneyt Tamer1,*, Ayris Salt2 and Gokhan Inat3
...ons were measured by the spectrophotometric methods. Data obtained revealed higher MDA levels in IPNV infected cells than negative control medium (p<0.001). However, SOD and GPx levels in IPNV infected cells were lover than negative control cells medium (p<0.001). These results indicate that, cell damage in RTG-2 cell line, that is caused by IPNV is assosiated with oxidative stress....
MUHAMMAD WAHEED MUSHTAQ1*, NAUMAN JAMIL KHAN1, NAVEED ASLAM DOGAR1, AISHA HAMEED2, ABDUL HAMEED2, MALIK ADIL2, HAROON IFTHIKHAR3 & SAIRA ISHAQ4
...n of Vit. C was measured spectrophotometrically by making a red color complex between 1, 10 Phenanthroline and Vit. C in the presence of ferric chloride (FeCl3). This colored product has maximum absorption at lambda max (λmax= 430 nm). Ascorbic acid was estimated from leafy vegetables placed at three different ...
MUHAMMAD ASLAM*, ASAD GULZAR, AMINA ASGHAR, MISBAH IRSHAD, ZAHRA NOREEN, SADAF JAVAID, ABRAR HUSSAIN, UMAIR AHMAD & SAMREEN KOUSAR
...d
precise UV spectrophotometric method by using 0.1N HCl as a
solvent, for the validation of levofloxacin in injection form. The λmax for
levofloxacin was found to be 293nm using 0.1N HCl as a blank.
Calibration curve data proved that the proposed method was linear in
the concentration range of 3 to 7μg/mL. The proposed method was
validated to determi...
Nauman Jamil Khan1, Rabia Tariq2, Hina Saleem2 and Muhammad Waheed Mushtaq2*
