Inamullah Khan, Muhammad Subhan Qureshi, Rajwali Khan, Syed Muhammad Sohail, Ijaz Ahmad, Muhammad Shoaib and Asim Ijaz
...also screen out. Overall sero-prevalence in cattle was 15.36% on SPAT, 14.39% by i-ELISA and 14.14% through PCR. Higher prevalence was recorded in abattoir shops (31.01%) followed by private farms (11%) and animal selling points (9.25%). Significant difference was recorded in different breeds i.e. achai, local non-descript and crossbred (non-descript x jersey) cattle. Higher prevalence in female cattle (15.14%) was recorded as compared to male (14%). Similarly...
Aziz-ul-Rahman, Farooq Yousaf, Naveed Anwar and Muhammad Abubakar
...seasons was recorded and sero-prevalence during summer was found to be higher (72.84% with GMT 20.18) than other seasons with an overall PPRV seroprevalence of 66.01%. Moreover, related to sex discrimination, 69.80:63.83% (GMT 14.83:15.92) antibody titre has been screened in female and male, respectively. So, based on the sero-prevalence, the evaluation of intrinsic (age groups and sex) and extrinsic factors (season fluctuat...
Ahmed Zein Mahmoud, Muaz Abdellatif and Ahmed Abdalla
...d a significantly higher sero-prevalence of 75.3% compared to 59.4% obtained from sheep, whereas camels were seronegative. The prevalence of PPR was increasing from 27.9% in 2011 to 77.3% in 2016. Seropositivity was higher in wet seasons (60.9 to 61.4%) to 56.7% in dry hot season. Species, year and location appeared to be having significant effect (p<0.01) on the frequency of circulating antibodies in the study. The results highlight that PPR in Hail is ala...
Hui Zhang2, Yajing Wang2, Kun Li2, Mujeeb Ur Rehman2, Fazul Nabi2, Rui Gui2, Yanfang Lan2 and Houqiang Luo1,2*
Amna Kausar1,2, Sana Anwar1, Naila Siddique2, Safia Ahmed1 and Javid Iqbal Dasti1,*
...omesticated bird species sero-prevalence was; broiler (n= 152; 60%), rural poultry (n=14; 6%), domestic desi birds (n=12; 5%), pigeons (n=9; 3.6%), desi chicken (n=9; 3.6%), broiler breeder (n=3; 1%) and layer (n=2; 0.8%). Overall sero-prevalence was 53% and average range of the hemagglutination inhibition (HI) antibody titer (MT log2) against AIV H9 remained 7±3. Real-time PCR results showed 8.3% (42/507) ...
Ali Zaman1,*, Abdul Haleem1, Sajjad Ur Rahman2, Adnan Amin3 Asma Saeed1, Sana Ullah4 and Naimat Ullah4
...f H5 antibodies. Highest sero-prevalence of Avian Influenza (H5) was recorded in district Abbottabad 36.15% (47/130) followed by Dera Ismail Khan 33.08% (43/130), Tank 26.92% (35/130), Peshawar 21.54% (28/130) and Mansehra 16.92% (22/130). Statistical analysis confirmed a significant (P<0.05) difference of sero-prevalence between the districts. There was a significant (P<0.05) association of occurrence of...
Bo Wu1, Hui Zhang2, Kun Li2, Khalid Mehmood2,7, Yan Zhao1, Bin Jiang1, Chengjun Xue3, Muhammad Tariq Javed6, Fazul Nabi2, Zhaoqing Han5,* and Houqiang Luo4,*
...taken to investigate the sero-prevalence and immunization program optimization of serotype O of FMD through ELISA and IHA in pigs in Zhejiang. A total of 368 serum samples were collected from June to July in 2016. Out of these samples, 23 (6.79%, 95% CI 4.4-9.9) pigs were found positive for FMD antibodies with the further distribution of 9.09% (95% CI 4.6–15.7), 6.90% (95% CI 2.6–14.4), 5.49%, (95% CI 1.8-12.4) and 4.35% (95% CI 0.9-12.2) from Wenz...
Qudrat Ullah1,*, Huma Jamil1, Zafar Iqbal Qureshi1, Muhammad Saqib2 and Heinrich Neubauer3
...anned to investigate the sero-prevalence of Coxiellosis and its relationship with some important risk factors in small ruminants kept at nine government livestock farms of Punjab, Pakistan. A total of 1000 blood samples (500 each from goats and sheep) were collected from animals kept at the respective livestock farms. An indirect-Enzyme Linked Immunosorbent Assay (iELISA) was used to detect anti-C. burnetii antibodies in the serum. Serological analysis ...
Abdul Kabir¹, Amjad Hussian Mirani¹, Jam Kashif1, Shumaila Manzoor2, Abdullah Iqbal3*, Ihsan Ullah Khan4 and Muhammad Abubakar2
...as performed to evaluate sero-prevalence of PPR in goats and sheep. The swab and tissue samples were collected from the surrounding areas from suspected sheep and goats to confirm the PPRV. Out of total 246 sera, 144 (58.54%) samples were found seropositive for PPRV. PPR was more prevalent in sheep (61.82%) as compared to goats (55.88%). In sheep population, the highest prevalence was recorded in the transhumant production system with 73.3% seropositive for PP...
Naseeb Ullah1, Assad Ur Rehman1, Aamir Khan1, Rabia Noushin2, Shahid Naiz Khan1, Syed Ishtiaq Anjum1, Sajida Afzal1, Asma Naseem1, Kenza Javed1, Ujala Rauf1 and Kalim Ullah1*
...tudy was to estimate the sero-prevalence of FMD in livestock in district Lakki Marwat, southern part of Khyber Pakhtunkhwa (KP) Pakistan. 376 blood samples were randomly collected from livestock (large and small ruminants) from December 2017 to April 2018 to examine the prevalence of FMD. The current study indicated that overall FMD in Livestock was 14.31% with 16.37% in small ruminants and 12% in large ruminants. A significant difference in sero-positivity wa...
Majeeda Rasheed1*, Tanveer Akhtar1, Nadia Mukhtar2, Muhammad Furqan Shahid2, Muhammad Imran3, Saima Yaqub2
Mushtaq Ahamd Khan1, Ziaul Islam2, *, Amin Ullah Jan3, Kamran Khan2 and Abdullah Shah3
...aled that overall 57.28% sero-prevalence was recorded in women of child bearing age. Highest (56.46%) seroprevalence was recorded in pregnant women as compared to non-pregnant women (43.53%). Highest (57.3%) sero- prevalence was recorded in women having 21-30 years age. Notably, the highest (25%) prevalence was reported in second trimester of pregnancy. Higher (52.6%) incidence of T. gondii infection was observed in illiterate women. The study demonstra...
Rao Muhammad Ramiz1, Arfan Ahmad2*, Aamir Ghafoor2, Muhammad Avais3, Qurat-ul-Ain1 and Muhammad Zahid Iqbal3
Muhammad Anwarullah1, Aneela Zameer Durrani1, Muhammad Ijaz1, Aftab Ahmad Anjum2, Muhammad Usman1*,Muhammad Zahid Iqbal1, Muhammad Husnain1,Ahsan Anjum3, Qurratulain Ashraf4 and Ghulam Mustafa3
...present study elucidated sero-prevalence of Mycobacterium avium sub sp. paratuberculosis (MAP) in cattle and buffalo. Diarrhea is a major risk factor for MAP, so 1100 blood samples were collected from diarrheic animals or having history of some diarrheic episodes from four districts of Punjab i.e. Kasur (n=300), Rawalpindi (n=230), Lahore (n=230) and Bahawalpur (n=340). These samples were tested using ELISA and overall seroprevalence found was 39...
Iqra Qazi1, Sohail Raza1*, Masood Rabbani1 and Muhammad Avais2
...inst BoHV-1. The overall sero-prevalence was 68.40%. Post pubertal animals (>3yrs) contributing more to disease occurrence. High sero-positivity was observed in buffalo (73.12%) as compared to cattle (60%) moreover, the sero-prevalence is higher in animals of greater size herds. Parity status is in agreement of strong correlation with BoHV-1 infection. Evidently, positivity rate was higher in animals bred with artificial ...
Olabode, H.O.K1, Mailafia, S1, Martha Echioda-Ogbole1*, Ameh, J.A1, Amadi, K.I1 and Meseko C.A2
...ults revealed an overall sero-prevalence of 5% with H5 and H9 haemagglutinating inhibiting antibody titre ranging from 1 log2-5 log2. Breed specific sero-prevalence were 4% and 16% for local and exotic (layers) chickens respectively, while broilers showed no detectable antibodies. Questionnaire responses indicated low awareness level on the zoonotic potential of AI but high level of awareness on the economic impact of the di...
Hussein Ali Naji1, Saad Hashim Al-Husseiny2, Zainab Abdul Hussein Saud3 and Wesssam Monther Mohammed Saleh1*
Qurat-ul-Ain1*, A. Ahmad2, Farhat Nazir Awan3, M. Rabbani1 and M. Hassan Mushtaq4
...s were collected and the sero-prevalence was estimated using an Ab capture ELISA assay. Inactivated BVDV vaccine was also prepared using local isolate (MK084980) and tested for induction of humoral immune response in comparison with commercial vaccine in experimental cattle calves. Calves of group A (n=5) and B (n=5) were vaccinated with self-prepared and commercial vaccines respectively followed by boosting 30 days post primary vaccination, while group C was ...
