Yating Cheng1, Wenlong Shi1, Xue Xiao2, Qirong Zhang2, Qihao Zhang1, Zhijian Su1, Qi Xiang1,2* and Yadong Huang1,2
...unit. In this study, two plasmids were used to produce an active human P4H tetramer (rhP4H) in Pichia pastoris. The P4Hα was cloned into pPICZαA, and PDI was cloned into PHIL. Real-time PCR showed that the P4H gene was expressed at the mRNA level. SDS-PAGE and western blotting analysis showed that the secreted expression of rhP4H was achieved successfully in P. pastoris GS115 cells. The catalytic activity of rhP4H was tested in a syst...
Basharat Ahmed1, Muhammad Hamza Azhar2, Farah R. Shakoori1 and Abdul Rauf Shakoori1,3,*
...llin+co-trimoxazole. The plasmids were observed in 15.1 % MDR strains of Campylobacter spp. which were found resistant to three or more antibiotics. Allthe strains contained a heterogeneouspopulation of plasmids ranging between 23.1 kb to 2.0 kb. grouped into seven different plasmid patterns. The plasmids (23.1 Kb) could only confer tetracycline resistance to the competent cells of ...
Muhamamd Rizwan1*, Muhammad Arshad2, Muhammad Kashif3, Aneela Zameer Durrani4, Asghar Abbas5, Tanveer Ahmad6, Muhammad Nadeem7 , Kinza Khan8
...e system against phages, plasmids and foreign genomic elements. Mostly prokaryotes uses their CRIPR Cas system to enhance the integrity of their cell membrane that inhibit the permeability of antimicrobials from host body into the bacterial cells. CRISPR Cas system also help bacteria to evade from the host immune system by suppressing the activity of their immune receptors e.g. TLR. CRISPR Cas system also help bacteria to attach with the host body and replicat...
Soliman*, Ahmed M.; Mahmoud**, Sabry Y. M. and Dawood*, Rehab A.
...in DH5a. The recombinant plasmids were obtained and sequenced. The nucleotide sequences were compared with corresponding viral nucleotide sequences reported in GenBank. The sequence analysis showed that; nucleotide sequence of OYDV-EG [Egyptian isolate (HM473189)] had 82-96% similarity compared with ten reported OYDV isolates. Thus, a method of identification and detection by RT-PCR of OYDV was established. This study also aimed to obtain OYDV-free plants from...
*El-Helaly, Sahar H.; ** Ahmed, Amal A.; * Awad, M.A. and ** Soliman, A.M.'
...in DH5a. The recombinant plasmids were obtained and sequenced. The nucleotide sequences were compared with corresponding viral nucleotide sequences reported in GenBank. The analysis showed that nucleotide sequence of the Egyptian isolate of AMV (GenBank Accession No. HQ288892) had percentage of similarity ranged from 97-98% with eight reported AMV isolates. Thus, a method of identification and detection by RT-PCR of AMV was established....
Amer, H.M.; Hussein, H. A.; El-Sabagh, I. M.; El-Sanousi, A. A. Saber,M.S. and Shalaby, M. A.
...t harbor the recombinant plasmids carrying N gene in correct orientation. Generation of recombinant baculoviruses was achieved by co-transfection of Spodopetra frugiperda (Sf-9) insect cells with a linearized replication-defective baculovirus DNA (Bac-N-BlueTM) and the transfer vector. The recombinant baculoviruses were plaque purified; verified for the presence of target sequences using PCR and propagated for generation of high-titer viral stocks. In vitro ex...
Bassma H. Elwakil1; Safaa M. Ali2*; Soad F. Hafez3; Adnan A. Bekhit4; Moustafa Y. El-Naggar5; Zakia A. Olama5
Novel Research in Microbiology Journal (2019), 3(6): 535-545
...between bacteria through plasmids or integrin. Different techniques including; phenotypic and genetic ones were used to evaluate the presence of β-lactamases among the isolated strains of K. pneumoniae. This bacterial species is the most commonly isolated pathogen (95 isolates) from all the examined samples (51.35%). Current results revealed that 30 and 14 strains of K. pneumoniae are positive to Extended spectrum β-lactamase (ESBL) production, and A...
Safaa M. Ali1*; Nadia A. Soliman2
Novel Research in Microbiology Journal (2019), 3(6): 590-597
...Bertani (LB) medium. The plasmids were isolated and then tested using universal primer (M13), to detect the fragment size and sequence for the Polymerase Chain Reaction (PCR) products. This study establishes an effortless and professional method for cloning of recent cellulase genes through ecological metagenomes. In the outlook, the metagenomic guide approachs may be functional to the elevated selection of novel cellulase from the environment....
Enas M. Hefzy1*; Reda M. Taha2; Safaa Abd El Salam2; Abdelrhman Abdelmoktader1; Mahmoud A.F. Khalil3
...-Pacific area. Virulence plasmids as well as other conjugal components contain the
genetic material that gives hvKp its hypervirulence phenotype. Although the vast majority of
hvKp isolates are antibiotic-susceptible, the incidence of virulent as well as resistant isolates,
such as carbapenem-resistant hvKp isolates, is continuously growing. Multidrug resistance
(MDR) and increased virulence of these strains may ...
Souad Oirdi Zahir1,2; Mounia El Khadir2,3; Dafr-Allah Benajah1,4; Sidi Adil Ibrahimi1,4; Laila Chbani5; Mohamed El Abkari1,4; Bahia Bennani1,2*
Novel Research in Microbiology Journal (2024), 8(4): 2542-2554
...stant strains. For that, plasmids of H. pylori 23S rRNA region harboring A2143G point mutation were constructed and used to develop polymerase chain reaction coupled with a high-resolution melting technique (PCR-HRM). Then, this method was applied on 233 gastric H. pylori positive samples. The obtained results showed that the developed PCR-HRM technique allowed specific identification of clarithromycin resistant and heteroresistant H. pylori samples, even if t...
