Syed Makhdoom Hussain1,*, Tasneem Hameed1, Muhammad Afzal2, Arshad Javid3, Nosheen Aslam4, Syed Zakir Hussain Shah6, Majid Hussain5, Muhammad Zubair ul Hassan Arsalan1 and Muhammad Mudassar Shahzad1
...p>-1) of microbial phytase on growth performance and nutrient digestibilityof Cirrhinus mrigala fingerlings fed sunflower meal based diet. The chromic oxide was added as inert marker in the diets. Three replicate groups of 15 fish (Average weight 5.05±0.013 g fish-1) were fed at the rate of 5% of live wet weigh and feces were collected twice daily. The results of present study showed improved growth performance of Cirrhinus mr...
Furqan Sabir1, Muhammad Tayyab1,*, Bushra Muneer2, Abu Saeed Hashmi1, Ali Raza Awan1, Naeem Rashid3, Muhammad Wasim1 and Sehrish Firyal1
...lementation of feed with phytase is the most suitable strategy for the availability of free phosphorus for the growth of monogastric poultry birds. Current study deals with the production and characterization of recombinant thermostable phytase from Thermotoga naphthophila (PHYTN). This study was an initial step for the fulfilment of domestic industrial requirement of phytase...
Naheed Bano* and Muhammad Afzal
... 1.5 and 3%) whereas the phytase levels were (0, 500 and 1000 FTU). The addition of 3 % citric acid and 1000FTU phytase produced significant interaction effect on calcium and phosphorus digestibility and different body contents of L. rohita. Phytase and citric acid increased the digestibility of calcium and phosphorus with improvement in body dry matter, protein, fat, energy and ash...
Muhammad Iqbal Anjum*, Shahbaz Javaid and Mukhtar Ahmad Nadeem
...-III contained R-II plus phytase Quantum® Blue @ 100 g/ton feed. Daily feed intake and weekly body weight gain per replicate were recorded for 35 days. On 35th day, blood and tibia bone samples from 5 birds per treatment were collected. Total weight gain and dry matter intake in chicks fed rations I, II and III were 1861, 1736 and 2023 gm/bird and 3242, 3284 and 3265 gm/bird, respectively. The chicks fed R-III got 16.00 and 8.00% higher weight g...
Muhammad Mudassar Shahzad1*, Syed Makhdoom Hussain1, Farhat Jabeen1, Abdullah Ijaz Hussain 2,Sajjad Ahmad3, Abida Ashraf4,Muhammad Zubair-ul-Hassan Arsalan1
...stimate the influence of phytase addition on mineral availability to fishfingerlings fed Moringa oleifera leaf meal (MOLM) based test diets. Anti-utritional factors (phytate) are present in plant by-products reduces the bioavailability of minerals to fish, resulting in poor fish erformance. Test ingredient was used as M.oleiferaleaf meal (MOLM) to prepare six experimental diets that were supplemented with graded levels of phytase
Muhammad Mudassar Shahzad1,2, Syed Makhdoom Hussain2,*, Farhat Jabeen2, Abdullah Ijaz Hussain3, Arshad Javid4, Muhammad Asrar2 and Muhammad Zubair-ul-Hassan Arsalan2
... FTU kg-1) of phytase. The fingerlings were fed at the rate of 4% of live fish body weight twice a day and faeces were collected from each tank. Present research work was carried out to determine the effect of phytase supplementation on mineral digestibility and carcass composition of Catla catla fingerlings fed MOSM based diets. Phytate in plant by-products decreases the bioavailability of minerals and dep...
Rana Zeeshan Habib1, Muhammad Afzal1, Syed Zakir Hussain Shah2,*, Mahroze Fatima3, Muhammad Bilal4 and Syed Makhdoom Hussain5
...nvestigate the effect of phytase (PHY) and citric acid (CA) supplementation on minerals digestibility of Labeo rohita fingerlings fed on canola meal based diet. Nine experimental diets were prepared by supplementing CA (%) and PHY (FTU/kg) at the level of 0, 0 (control); 0, 1000; 0, 2000; 1.5, 0; 1.5, 1000; 1.5, 2000; 3, 0; 3, 1000; 3, 2000 respectively. Chromic oxide was used as inert marker in diets to estimate mineral digestibility. Fish fed on PHY s...
Kalimullah Khan1,*, Hafsa Zaneb2, Zia Ur Rehman3, Hamza Maris4 and Habib ur Rehman1
... that supplementation of phytase enzymes affected the weight and length of small intestine and feed conversion ratio (FCR) and did not affect the other zoo technical variables like body weight, feed consumption, and weight gain during the whole experimental period. Application of phytasefailed to exert any influence on the weights of gizzard, provetriculus, heart, liver, spleen and empty intestine. The weights and lengths of...
Asad Sultan1, Rabia Ali1, Rifat Ullah Khan2,*, Sarzamin Khan1, Naila Chand1 and Ambrina Tariq3
...y assessed the impact of phytase on enhancing the nutrient retention and phosphorus utilization in two different sorghum cultivars fed to broiler. In an open sided house, a total of 160, 14-days-old broilers were allotted to four dietary bioassay treatments and fed mash red and white sorghum grain with or without phytase enzyme (500 FTU/kg) for 12 days (including 4 days adaptation period). Standard lab protocols were adapted...
Muhammad Afzal1, Nighat Sultana1, Ali Hassan1, Syed Zakir Hussain Shah2,*, Mahroze Fatima3, Syed Makhdoom Hussain4, Muhammad Bilal5 and Majid Hussain2
...tric acid (CA) acidified phytase (PHY) pretreated soybean meal (SBM) based diet.Basal SBM based diet was supplemented with two levels of CA (0 and 2%) and two levels of microbial PHY (0 and1000 FTUkg-1) in factorial arrangement resulting in the formulation of four experimental diets. Chromic oxide (1%) was added as an inert marker in the diets to evaluate the nutrient digestibility. Important water quality parameters such as temperature, pH and diss...
Muhammad Mudassar Shahzad1*, Syed Makhdoom Hussain2, Afia Muhammad Akram1, Arshad Javid3, Majid Hussain5, Syed Zakir Hussain Shah4 and Asma Chaudhary1
...o evaluate the effect of phytase supplementation on growth and nutrient digestibility of the Catla catla fingerlings fed Moringa oleifera leaf meal (MOLM) based diet. The phytic acid’s presence in plant by-products decreases the bioavailability of nutrients to fish, resulting in poor fish growth and low nutrient digestibility in the body. Experimental diet was divided into six groups and were supplemented with graded levels (0, 300, ...
Muhammad Afzal1, Mahroze Fatima2, Aasma Qamar1, Muhammad Farhan1 and Syed Zakir Hussain Shah3,*
... of citric acid (CA) and phytase (PHY) pre-treatment on growth, muscles proximate composition, whole body proximate composition and minerals status of Labeo rohita juveniles. Four experimental diets were prepared by supplementing citric acid (0 and 2%) and phytase (0 and 1000 FTU/kg) in 2×2 factorial arrangements. Total 180 juveniles (3.45±0.013) were distributed to 12 tanks having triplicate tank for eac...
Syed Zakir Hussain Shah1*, Muhammad Afzal2, Mahroze Fatima3, Syed Makhdoom Hussain4 and Tanveer Ahmed5
...mentation (3 levels) and phytase (PHY) supplementation (3 levels) as the main effects. CA was supplemented at 0, 1.5 and 3% and PHY was added at 0, 750 and 1000 FTU/kg diet in a 3 × 3 factorial arrangement. All the nine test diets were contained 37.6% crude protein and 4.72 kcal/g gross energy. Results showed improved (p<0.05) dry matter, crude protein, crude fat and gross energy digestibility by fingerlings when fed PHY sprayed diet. Similarly...
Nuzhat Naseem, Sajid Abdullah and Sana Aziz*
...fish because there is no phytase activity in agestric fish. This study was performed to determine the impact on proximate composition of whole-body of Labeo rohita (fingerlings) fed distiller’s dried grains with solubles based diet, supplemented with phytase. Six different feeds were formulated by supplementing different concentrations of phytase. i.e. D1 without
Ali Raza1, Muhammad Sharif1*, Fawwad Ahmad1, Asghar Ali Kamboh2, Muhammad Saeed3, Muhammad Ashraf1 and Asad Ullah Hayder1
... effect of vitamin D and phytase on performance, blood mineral level and slaughter parameters in broiler chicken. Diet A was without vitamin D and phytase supplementation (control). Diets B, C, D, E, F and G were supplemented with phytase at the rate of 400 FTU/kg, 800 FTU/kg, vitamin D 4000 IU/kg, 8000 IU/kg, phytase 400 FTU/kg + vitamin D 4000 IU/kg an...
Muhammad Bilal Munawar1, Saima Naveed1, Shafqat Nawaz Qaisrani1, Jibran Hussain2, Shahzad Munir3, Ayesha Ahmed3, Mubbashir Hussain4, Ahmad Kamran Khan5, Abdullah Jalal6 and Irfan Ahmed7*
...of post pelleting liquid phytase supplementation was evaluated in broilers on the basis of growth production, plasma and tibia phosphorus. The (n=504) day-old broiler chickens procured randomly allocated into 6 treatments dietary treatments were: B positive control (PC) diet (0.42% available phosphorus in grower feed, A negative control (NC) diet with (0.30% avail. P in starter and 0.26% avail. phosphorus in grower) while four diets C, D supplemented with NC+ ...
Aqsa Javaid, Masoom Yasinzai* and Khunsa Saeed Kiyani
... was the optimization of phytase production from Bacillus subtilis KT004404 through statistical model (Response Surface Methodology) by utilizing centrally composite design (CCD). The optimal phytase activity obtained after CCD design was 132.32 U/mL at 35 °C, pH 6.5, inoculum density of 2.5mL after incubating for 24h at 75 rpm. Extracellular enzymes were then partially purified in 2 steps (ammonium sulfate precipitati...
A 90-day feeding trial was designated to evaluate how canola meal (CM)-based diet supplemented with phytase (PHY) and citric acid (CA) affects whole-body composition and hematological parameters in Cirrhinus mrigala and Cyprinus carpio fingerlings. Sixteen experimental diets (T1─T16) with varied CA (0 and 2.5%) and PHY (0 and 750 FTU/kg) levels were prepared. The fingerlings were fed at the rate of 5.0% of live fish body mass. Fifteen fish were randomly stocked into triplicate tanks for each of sixteen test diets. Significant (p<0.05) increase in crude protein (CP) and crude fat (CF) was noticed in fish fed T12 (2.5% CA and 750 FTU/kg PHY) diets. Comparison of treatments showed maximum values of RBCs (3.57×106 mm-3 and 3.18×106 mm-3), PLT (83.32 and 80.35), Hb (8.92g/100ml and 8.62g/100ml) and PCV (29.07% and 30.07%) in C. mrigala and C. carpio fingerlings, respectively, when fed with T12 diets. Conclusively, a 50% CM-based diet with 2.5% CA and 750 FTU/kg PHY supplementation, performed better in terms of whole-body composition and hematological parameters in C. mrigala and C. carpio fingerlings.
...d diet supplemented with phytase (PHY) and citric acid (CA) affects whole-body composition and hematological parameters in Cirrhinus mrigala and Cyprinus carpio fingerlings. Sixteen experimental diets (T1─T16) with varied CA (0 and 2.5%) and PHY (0 and 750 FTU/kg) levels were prepared. The fingerlings were fed at the rate of 5.0% of live fish body mass. Fifteen fish were randomly stocked into triplicate tanks for each of sixteen test diets. Significant (p<...
Aaqil Muhammad1*, Asad Sultan1, Sarzamin Khan1 and Umer Sadique2
...e effect of protease and phytase in a sorghum-based diet on nutrient bioavailability and performance indices of broilers. A total of 160-day-old broiler chicks were purchased from the local hatchery and assigned to four treatment groups replicated four times with 10 birds per replicate in a completely randomized design. There were four dietary treatments designated as control contained 100% maize-based diets without sorghum and enzyme, HDV-100+protease (T1) ha...
Yana Gocheva*; Stephan Engibarov; Rumyana Eneva; Irina Lazarkevich; Simona Mitova; Ivanka Boyadzhieva
Novel Research in Microbiology Journal (2024), 8(6): 2772-2789
...e diversity of bacterial phytases, their importance in increasing the availability of phosphorus and other nutrients necessary for the growth and development of various plants and animals, and their roles in maintaining environmental sustainability. Phytases, enzymes that catalyze the hydrolysis of phytic acid, play a major role in various biotechnological processes; especially in agriculture. Among the diverse sources of
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