Effect of Phytase, Protease, and their Combination on the Nutrients Bioavailability and Performance Indices of Broilers Fed a Sorghum-Based Diet Under Local Climatic Conditions of Khyber Pakhtunkhwa Pakistan
Effect of Phytase, Protease, and their Combination on the Nutrients Bioavailability and Performance Indices of Broilers Fed a Sorghum-Based Diet Under Local Climatic Conditions of Khyber Pakhtunkhwa Pakistan
Aaqil Muhammad1*, Asad Sultan1, Sarzamin Khan1 and Umer Sadique2
ABSTRACT
The present study evaluated the effect of protease and phytase in a sorghum-based diet on nutrient bioavailability and performance indices of broilers. A total of 160-day-old broiler chicks were purchased from the local hatchery and assigned to four treatment groups replicated four times with 10 birds per replicate in a completely randomized design. There were four dietary treatments designated as control contained 100% maize-based diets without sorghum and enzyme, HDV-100+protease (T1) had 100% sorghum without any maize and supplemented with protease enzyme; HDV-100+phytase (T2) had 100% sorghum without maize and supplemented with phytase enzyme; HDV-100+protease+phytase (T3) had 100% sorghum without maize and supplemented with the combination of protease and phytase enzyme. The result showed improved body weight gain and feed conversion ratio during the overall experimental period in T1, T2, and T3 diet groups than in the control group. During the entire experimental period feed intake and livability remind unchanged. On days, 21 and 28 of the experimental period, the litter moisture content was not effected but at day 35 of the experimental period, the litter moisture content was calculated significantly higher in the T2 and T3 diet groups than in the control and T1 diet groups. Relative organ weight was not effected throughout the experimental period. The crude protein and apparent ileal metabolizable energy were calculated significantly higher in the T3 diet groups as compared to the remaining groups. Overall, the digestibility of essential amino and non-essential amino acid were recorded (P<0.05) higher in the T1, T2, and T3 diet group as compared to the control group. In conclusion, the replacement of maize with sorghum in combination with exogenous enzyme protease and phytase can effectively improve the growth performance and nutrient digestibility in the broiler.
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