Farah Mansha, Abdul Rehman*
...selected on the basis of chitinase assay and diameter of zone of hydrolysis. All the isolates were identified as Bacillus sp. on the basis of biochemical tests, and showed optimum growth at 37°C and pH of 7. All the bacterial isolates showed highest enzyme activity at pH 7. Isolates H4 and M4 showed maximum enzyme activity at 55°C while H1 showed highest activity at 70°C. Chitinase activities exhibited by ...
Farah Deeba, Hafiz Abdullah Shakir, Muhammad Irfan, Javed Iqbal Qazi*
Chitinase production in organisms: a review
...Chitinase are chitin degradable enzyme have control of phytopathogens, physiological functions and degradation of chitinous waste. Interest in chitin wastes utilization is increasing day by day because of its natural resistance against degradation. The review focused on different sources of naturally chitinase production in organisms was discu...
K. Abbasi 1 , R. Wick2† and D. Zafari1
Chitinase activity of biocontrol fungi cultured from the golden potato cyst nematode, Globodera rostochiensis
...their ability to degrade chitin by the N-acetylglucosamine-dinitrosalicylate method. Ten isolates with high chitinase activity were selected for further characterization. Of the 10 selected isolates, three chitinase enzymes were evaluated using three substrates. Based on the results of our chitinase assay, Beauveria bassiana, Lecanicillium muscarium, Pae...
Hesham M. Abd El Halim1, Maysa M. Hegazy1,2, Aishah Alatawi3, Imtiaz Ali Khan4 and Munawar Saleem Ahmad5*
...ve long been known to be chitin inhibitor and agents for developmental arrest, chitinase genes and genes that are vital for starting the differentiation program of cells at discrete steps during development are major targets for biocide plant extracts. The current study evaluates the in vitro toxic effects of golden piller (Cupressus macrocarpa) and galangal (Alpinia officinarum) against Spodoptera frugiperd...
Mohsen M. Elsharkawy1, Salem H. Homayed1, Mohamed M. Elsawy2 and Amr A. Khedr1
...hogenesis related genes, chitinase, PAL1 and LOX1.
Conclusion: In cucumber, treatments with artificially generated wind and root colonizing F. equiseti
GF19-1 or its CF elicited induced resistance against CMV infection, leading to a restriction of
pathogen propagation and disease development.
...
Shimaa A. Khalaf1 , Mohsen M. Elsharkawy2, Samir A. Sidaros2, Shawky A. El Kewey2, Ayman F. Omar2, Ali Hamed1
Farah Deeba1, Hafiz Abdullah Shakir1, Muhammad Irfan2, Muhammad Khan1 and Javed Iqbal Qazi1*
...strains were isolated on chitinase producing medium (CPM) from soil samples collected from local termites’ influenced areas. Of all these, three isolates gave positive test for chitinase screened on the bases of clear zone on CPM following chitinase assay. The best chitinase producer was selected and identified as Bacillus subtilis employing...
Blood Profile of Soybean Meal Substitution with Black Soldier Fly Larvae in New Zealand White Rabbit
Ardi Prasetio1*, Christina Maria Sri Lestari2, Sutaryo Sutaryo2, Manar Fayiz Mousa Atoum3,4, Muhammad Zahoor5, Asma Nisar6 and Muhannad Illayan Massadeh7
...id profile. However, the chitin contained in BSLF may cause subclinical inflammation by blood parameter detection. Thus, this study investigated the BSLF substitution effect on rabbit blood traits. This study was performed using 28 wk old weaned male New Zealand White rabbits (1300 g ± 130 g) that were randomly divided into control group (T0) and treatment group (T1, T2, T3), which respectively substituted 0 %, 10 %, 20 %, and 30 % SBM with BSFL. Blood ...
Ali Ahmad1*, Zubair Aslam1, Korkmaz Bellitürk2, Ehsan Ullah1, Ali Raza1 and Muhammad Asif3
...eases, lipase, amylases, chitinase), which degrade organic substrates even after they have been secreted. Earthworms change the chemical and physical characteristics of organic substrates, over time reducing the C:N ratio, increasing the surface area exposed to bacteria, and improving the microbial degradability of organic materials. This study aims to demonstrate how organic amendments, rather than chemical fertilizers, might improve soil health and plant dev...
S. Ahmed†, Q. Liu and H. Jian
...bolite with protease and chitinase
activity was used as seed bacterization in green house experiments. In green house significant reduction in white
female development was observed for avermectin (84.53%) followed by B. cereus strain B48 (78.10%) as compared
to results of the untreated control treatment (P≤0.05).
...
A.H. Choshali1, S. Rezaee2, S. Jamali3, H. Reza3, Zamanizadeh4 and F. Rejali5
...quantitative activity of chitinase and ß 1, 3 glucanase enzymes was assessed on 2, 4, 6 and 8th days after M. incognita inoculation based on split- plot in time design. Also the results showed that AMF pre-inoculation caused a significant decrease in RKN pathogenicity factors (number of galls, eggs, egg sacs and J2) in both tolerant and susceptible cultivars. Inoculation of susceptible roots with AMF significantly reduced the nematode pathogenicity facto...
Md. Rashedul Islam1, Abul Farah Md. Hasanuzzaman1*, Md. Latiful Islam2, Ghausiatur Reza Banu1
...n in captivity. But some chitinolytic bacteria (Vibrio spp., Aeromonas spp., and Pseudomonas spp.) poses major threats in the production line of mud crab larvae. Hence, the present study tried to determine prevalence and load of total and pathogenic bacteria in the different components of the mud crab hatchery. Selective agar media, a series of biochemical tests, and bacteria isolation kits were used for the isolation and identification of bacteria. The genus ...
Burarah Arooj1, Zeeshan Mutahir1, Moazzam Ali1, Mohsina Akhter2, Malik Siddique Mahmood1, Arslan Hamid3 and Mahjabeen Saleem1*
... enzyme that breaks down chitin polymer’s β-1, 4-glycosidic bonds. Chitinases having high thermostability makes them suitable to use in different industrial sectors. A 1464-bp chitinase gene, from Paenibacillus sp. Y412MC10 was cloned and produced in E. coli BL21 cells using the pRSETB vector. Gel filtration and ion exchange chromatography were used to purify the recombinant 52 ...
Yunilas1*, Muheri Indra Aja Nasution2, Edhy Mirwandhono1, Adi Fathul Qohar3
...al phase (BSFP) there is chitin which is a limiting factor in livestock rations because the bodies of poultry and monogastric livestock cannot digest it. Chitin is found in the BSF exoskeleton which is bound to proteins, minerals, and pigments, so it is necessary to do processing to reduce the chitin content first by fermentation using organic acids. Therefore, this study aimed to determin...
M.A. Abo-EInasr l, Kh.A. EL-Dougdougl, M.H. El-Kattan2 and L.A. Salem2
...tivity of peroxidase and chitinase enzymes. Salicylic acid (0.5%) and potassium sulfate (3%) gave the highest effect in inducing SAR. where the treatment with them had forbid disease symptoms, and virus concentration was (0), 25 days post inoculation. In addition, the biochemical changes reached to the maximum values. whereas other chemicals gave a medium ability to induce SAR and gave varied values....
M. Ismail Chaudhry and Hanif Gul
... 25% W.p.
which inhibit chitin sythesis in insects were tried
against 2nd and 3rd instar larvae of shisham defoliator,
Plecaptera refexa Guen. (Notodontidae). Both the
chemicals in laboratory as well as field trials, in
doses of o.02 and 0.04% concentrations gave cent percent
mortality of the larvae. The residual effect of the
insecticides continued up to about one month and
provided cover for two successive and overlapping
generations the non-...
M. Ismail Chaudhry and Bashir Hussain Shah
...us thuringiensis and chitinase, were conducted against larvae of poplar defoliator, Ichthyura anastomosis Steph. in the laboratory. In the first trial there was 100% mortality of larvae within three days of release on poplar shoots treated with 1% and 1.5% concentrations of Dipel and within two days in case of 2% concentration of Dipel. In check there was only 36% natural mortality of larvae. In the second trial there was 100% mortality of larvae wi...
Eman Abdelrazik1, Sahar H. Abdel-Baset2*, Abdelghafar M. Abu-Elsaoud3,4 and Shimaa M.A. Mohamed5
...ylalanine ammonia-lyase, chitinase, β-1,3 glucanase, and total phenolic activities. The integration of several techniques, including fulvic acid, compost, and cattle manure, might have a substantial effect on enhancing peanut development, biochemistry, and the ability to resist and control M. arenaria....
