Bin Wang1, 2, Qianji Ning1,*, Qian Wang2, Wei Peng2, Tong Hao2,*and Jinsheng Sun2,*
...nalyzing the subcellular annotation of the molting interaction network, the subcellular localization of 12 proteins was obtained. Half of these proteins located on the cell membrane, indicating the close relationship between molting process and the changes of membrane. The results of this work provide a theoretical basis of the further elucidation of the molting mechanism for E. sinensis.
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Binbin Shan, Yan Liu, Changping Yang, Shengnan Liu and Dianrong Sun*
Lei Wang1, Lu Yang2, Lei Luo1, Yaqi Gao1*, Jian Gao1 and Ping Liu1
...G, and GO for functional annotation and classification. Some differences in the regularity of distribution and the time of eclosion of the pupa were detected between the southern and the northern populations. We obtained 142.65 Gb of data from transcriptome sequencing and recovered 70,397 unigenes through a de novo assembly. In total, 2089, 2420, and 6286 differentially expressed genes were gained from comparing groups of male adults, female adults, and...
Rabia Saeed1, Muhammad Naveed1, Muhammad Razaq2*, Muhammad Rafiq1, Muhammad Tahir Jan1, Syed Ishfaq Ali Shah1, Shabana Wazir1 and Fahad Munir3
...n family. In the present annotation, we record for the first time A. coquebertii in cotton growing areas of Dera Isamil Khan, Muzafar Garh and Multan districts, Pakistan.
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Zilu Zhang1, Ning Wang2, Ce Hou1, Laiba Shafique1, Saif ur Rehman1, Zhuyue Wu2, Zhiqiang Wang1, Mingsong Wei3* and Kuiqing Cui1*
...kb sequence. The further annotation results showed that a tRNA was located at 4218-4290bp, STR1 (TGTGTGTGTGTG) and STR2 (ATATATATATAT) were located at 9473-9484bp and 12451-12462bp, respectively. Three micro RNAs, dme-mir-1, dme-mir-263a and cbr-mir-354 were confermed to locate at 3002-3023bp, 9078-9094bp and 10866-10882bp, respectively. There is a CpG island at 4390-4540 bp. Our results showed that the PIS regions have no effect on polled phenotype of Guanzho...
Umer Farooq1,2, Nimra Murtaza2, Abubakar Siddique1, Bilal Saleem1, Obaid Ur Rehman1, Nageen Zahra1, Muhammad Uzair1, Muhammad Naeem Riaz1,3* and Muhammad Ramzan Khan1*
...alignment and functional annotation to identify deleterious non-synonymous SNPs (nsSNPs) in cattle breeds of Pakistan. For this purpose, genomic data of four different purpose cattle breeds including Bhagnari, Cholistani, Sahiwal and Red Sindhi was analyzed. Comparison with taurine reference genome ARS-UCD.1.2.99 discovered 29,032,662 genomic variations of which 25,469,157 were single nucleotide polymorphisms (SNPs) and 3,563,505 were Insertion/Deletions (InDe...
Jie Wang1, Di Fang1, Jianqing Zhao1, Fei Huang2, Bo Liu2, Weikun Tao2, Baoshan Cui2 and Qinghua Gao1,2,3*
...e analyzed by functional annotation and related bioinformatics analysis. We found that Q30 percentage range of eight samples was 91.79-92.37%. The filtration sequence was 44106250-54234844. Compared with the reference genome by TopHat software, the net reading ratio of the bovine reference gene at each stage was 93.17-9 4.23%, the ratio of sequence numbers to multiple sites of the genome was 2.99-4.89. The DEG was identified by using the fold change ≥2 and ...
Wei Fang1,2,3, Jiawei Hong1,2,3,4, Zhengyi Fu1,2,3, Jing Hu1,2,3, Gang Yu1,2,3, Zhenhua Ma1,2,3* and Humin Zong5*
...ed to perform functional annotation classification, metabolic pathways and SSR feature analysis. The results showed that a total of 42.40 Gb data were obtained from transcriptome sequencing, and 38,817 Unigenes were obtained after assembly and de-redundancy; Using BLAST to annotate the assembled unigenes with seven functional databases (NR, NT, GO, COG, KEGG, Swissprot and Interpro), a total of 23,417 unigenes were annotated (accounting for 61.14%); A total of...
Hui Fang*, Kai Xue and Teng Yang
...ion of ADPKD. Functional annotation and enrichment analysis of DEG were performed. iRegulon was used to construct the miRNA-mRNA network and predict related transcription factors (TFs) associated with ADPKD. The function of miRNA network was explored by analyzing GO and KEGG enrichment. Further, qRT-PCR was performed in vitro to determine the expression of miRNAs and mRNAs associated with ADPKD. In the results, 2 DEMs and 1090 DEGs were identified. While DEGs ...
King Hei Ip
..., the incomplete genetic annotation of all the bat species, a lack of understanding of bat-virus interactions and viral co-infection, and biases in bat-virus relationship studies hinders the understanding towards bat antiviral innate immunity. By completing the genetic annotation of bats, developing wild-like environments when studying the bat immune system, improving data collection methods and conducting further studies on...
Ye-Ling Lao, Jin-Long Huang, Cheng-He Sun, Xiao-Ying Huang, Ting Wu and Qun Zhang*
...d MitoFish were used for annotation. We then successfully annotated the mitogenome of M. altispinosus, which has a total length of 16,767 bp. The gene composition and base preferences of M. altispinosus are similar to those of other New World cichlids. Based on the concatenated sequences of 13 protein-coding genes and two rRNAs from the mitogenomes of 37 New World cichlids and one African cichlid, we reconstructed phylogenetic trees using maximum likelihood an...
