Articles

Lessons to Learn from the COVID-19 Pandemic: Recent Advances in mRNA Vaccines Against Viral Diseases

Mahmoud M. Bayoumi

The most effective way to prevent infectious viral diseases is through vaccination. The use of mRNA technology in vaccine development has proven to be a highly effective approach that can be utilized to rapidly develop vaccines against infectious viral pathoge…

Occurrence and Distribution Patterns of Canine Parvoviral Enteritis in Abuja, Nigeria

Iko-Ojo Charity Ikwe Agada, James Agbo Ameh, Olatunde H. Olabode, Martha Echioda-Ogbole

Canine parvoviral enteritis (CPE) is a viral disease of dogs caused by carnivore Protoparvovirus 1 (CPV). The CPV is a small, non-enveloped, single-stranded DNA virus of the family Parvoviridae. CPE is a highly contagious enteric disease of dogs transmitted ma…

Virologic Detection and Molecular Characterization of Rabies Virus in Delta Region, Egypt

Morcos Ibrahim Yanni, Eid Elsaid Abdelaziz

Rabies is a fatal worldwide viral disease and contributes to a great economic load in Egypt through the death of animals and control strategy costs. Due to Egypt’s privileged location and friction with neighboring countries and far countries from Europe…

Cocktail Phage Therapy for Bacteria Contaminating Meat in Egypt

Amany M. Reyad, Aya Maher Rabie, Reda Mohamed Taha, Khalid El-Dougdoug

Bacteriophages have crucial advantages over conventional methods of controlling pathogenic bacteria in which they have a high ability to self-reproduce, host specificity, and develop with their bacterial hosts. The most common pathogenic bacteria were isolated…

Improving the Phytosanitary of Fig Plants Infected with Fig Latent Virus by Nanochitosan and Biomagic

Hanaa H.A. Gomaa, Dalia Y.A. Amin, Mona A. Ismail, Basma Hamdy, Khaled A. El-Dougdoug

Fig viruses have been found as naturally mixing infection in many countries in the world and Egypt. The present study aimed to evaluate the potential effect of nanochitosan and biomagic for treatment of the pathological effects related to virus infection in fi…

Molecular Characterization of Recent Field Isolate of Lumpy Skin Disease Virus Compared to the Vaccinal Strains

Akram A. Salama, Moustafa A. Zaghloul, Ahmed A. Zaghawa, Mohamed A. Nayel, Ahmed M. Elsafey, Mohamed F. Azooz et al.

The lumpy skin disease virus (LSDV), causes lumpy skin disease (LSD), is a transboundary viral disease of cattle that has a significantglobal economic impact. This study aims to identify the lumpy skin disease virus that circulate…

Sequencing and Phylogenetic Analysis of GPCR, RPO30, P32 and EEV Glycoprotein Genes of Lumpy Skin Disease Virus Recent Isolates in Egypt

Moustafa A. Zaghloul, Mohamed F. Azooz, Saleh E. Ali, Heba M. Soliman, Maha M. Sayed, Mohamed H. Kafafy et al.

Lumpy skin disease is an emerging viral illness of ruminants that is highly contagious and economically damaging. The goal of this study is to perform sequencing and phylogenetic analysis of GPCR, RPO30, P32 and EEV glycoprotein genes of Lumpy Skin Disease Vir…

Effect of environmental factors on Peanut Mottle virus infection and population of insects on Lentil in Egypt

 Peanut Mottle Virus (PMoV) was isolated from Lentil cvs. Giza51. The infected indicator plants featured severe mosaic on Lentil cv. Giza51, mottling on cv.Giza9, mild mottle on cv. Giza 4, yellow mild mottling on cv.  Giza370. Seed transmission test…

Phylogenetic relationship of some Potyviridae members that found in Egypt.

Mervat M. Fath- Allah, Amal A. Ahmed, Hala A. Amin

Five different plant samples showing different Potyviridae symptoms were previous isolated and identified. All infected plant samples were examined using ELISA using against, number of potyviruses polyclonal antibodies. The ELISA results gave a positive reacti…

Characterization of very Virulent Infectious Bursal Disease Virus in Egypt during the Period 2019-2020

Mai M. Dawood, Mohamed Fawzy, Mohamed S. Elshahidy

Infectious bursal disease virus (IBDV) is a highly contagious disease of young chickens, that results in bursal necrosis and severe damage of the immune system. IBDV remarkably persist to affect the poultry industry in Egypt. Hence this study was done to ident…

Characterization of Tobacco Rattle Tobravirus from Kaki (Diospyros Kaki)

Saiwa N. Zein

Kaki (Diospyros kaki) trees, showing vein banding. upward rolling of leaves and stunting of growth were observed in the farm Of South Tahrir. Isolation trials revealed that these symptoms were induced by Tobacco rattle virus. The virus was transmitted by mecha…

Identification of Sugarcane Mosaic Potyvirus Strains in Egypt

A.l. Abd El-Fattah, A.s. Saclik M.M. El-Kholi, I.A. Åbdcl-Hmnid, M.A. Madkour

Sugarcane mosaic pot.1Virus (SCMV) is the causal agent Of mosaic disease which is reported to be one of the most important viral diseases attacking sugarcane crop in Egypt. Therefore. the present work was designed to screen the SCMV strains in Egypt based on b…

Immunocapture Polymerase Chain Reaction (IC-PCR) and Nucleic Acid Hybridzation Techniques for Detection of Spiroplasma citri

Om-Hashem M. El Banaa l, A.A. Abou Zeid, Fawzia I. Moursy, Azza, G. Farag

Spiroplasma citri Saglio et al is the type species of the genus spiroplasma. Spiralin gene is the gene responsible for spiralin which is the major protein of Spiroplasma citri (S. citri Saglio et al), the causal organism of citrus stubborn disease. RecA gene p…

Biological and Molecular Characterization of Different Isolates of Potato Virus Y (N Group)

A.Y. Ahmad, M. A. Amer, T. A. Mostafa, F. M. Abo El-Abbas, M. El-Hammady

Ten isolates of Potato virus Y (PVY) were collected from diseased potatoes grown at different locations in Egypt and some of their biological and molecular characters were studied using several techniques. Many different symptotypes were appeared on Chenopodiu…

Large Scale Production of Potato Cirus Y Necrotic Strain (PVYN) Coat protein through Expression the CP Gene in E. coli

A.S. Gamal El din, Sohair I. El-Afifi, A. S. Sadik, Nashwa M. A. Abd El-Mohsen, H. M. Abdelmaksoud

A fragment with a size of 801 bp from the CP gene was amplified from Potato virus YN isolated from potato plants cultivated at Monofyia Governorate. RNA was extracted from virus-infected tissues of D. metel leaves by the use of degenerate primers through polym…

Human Papillomavirus in Spontaneous Abortion

Enas K. Abo El-Maged, Azza H. El-Salakwy, Safia A. El-Gamal, Gehn Allam

First trimester spontaneously aborted products of conception were obtained from 100 Egyptian women. Placental tissue specimens from 100 women of full term normal vaginal deliveries were used as a control group. Their age ranged from 17-42 years (mean = 29.31).…

Identification of A Virus From Naturally Infected Garlic Plants

S.A. El-Kewey, R.A. Omar, S.A. Sidaros, Samaa Abd El-Khalik

The isolated virus was identified according to host range, symptomatology, differential hosts, virus stability, modes of transmission, and electron microscopy. The virus has a narrow host range. Dilution end point was found to be 10-2. Thermal inactivation poi…

Molecular Characterization of Potato Spindle Tuber Viroid Egyptian Isolate

E. K. Allam, Kh. A. El-Dougdoug, M. A. Amer, A. A. Abou-Zaid, S. M. Amin

Potato spindle tuber viroid (PSTVd) was detected in potato plants grown in Egypt using return-polyacrylamide gel electrophoresis (R-PAGE) and molecular techniques. The method involved the extraction of circular nucleic acids followed by electrophoretic analysi…

Molecular Cloning and Expression of the Coat Protein Gene of Plum Pox virus El-Amar Strain in E. coli

M. A. Abou El- Nasr, Kh. A. Dougdoug, Hayam S. Abdelkader, Rehab A. Dawoud

Plum pox potyvirus (PPV), the causal agent of Sharka disease of Prunus inflicts severe crop losses in affected El-Amar area. The virus isolated from EL-Amar apricot trees, was propagated on apricot healthy seedlings. Degenerated oligonucleotide primers were de…

Cloning, Expression and Nucleotide Sequence of Coat Protein Gene of an Egyptian Isolate of Potato Virus y Strain NTN Infecting Potato Plants

M. A. Amer, M. H. El-Hammady, H. M. Mazyad, A. A. Shalabyl, F. M. Abo-El-abbas

Reverse transcription- polymerase chain reaction (RT-PCR) amplified product of the expected size of 801 bp was cloned into the Pinpoint Xa-l protein expression vector. The accury of each PCR amplified PVY CP gene was tested by PCR, restriction analysis, and tr…