Kaki (Diospyros kaki) trees, showing vein banding. upward rolling of leaves and stunting of growth were observed in the farm Of South Tahrir. Isolation trials revealed that these symptoms were induced by Tobacco rattle virus. The virus was transmitted by mechanical inoculation. Symptoms were reproduced in the greenhouse on D.Kaki seedlings and tested host plants. Host range of the virus studied was restricted in Chenopodiceae. Cucurbitaceae. Ebenaceae. Leguminosae and Solanaceae. The identity of the virus to TRV was confirmed by mode of transmission. particle morphology and serological typing. Examination or the purified virus preparation 1» electron microscopy revealed rod-purify the Egyptian isolate of TRV. The yield of the virus was 2.6-5.7 mg/ 100 g tissue of Nicotiona rustica plants. The polyclonal antibodies raised against the Egyptian isolate of TRV had a specific titer of I: 10000. The concentration of 12G and lgG conjugated with alkaline phosphatase was I : 1000. The prepared antiserum was used for detection of T RV using dot-blot immunobinding assay (DBIA). This is the first report for isolation of Tobacco rattle virus Tohrarirus (T R V) from D.Kaki under the Egyptian conditions.