Articles
308 open-access articles · page 9 of 16
Research ArticleReverse Transcripion-Polymerase chain Reaction Mediated Detection and Identification of Tobacco mosaic virus- Egyption Strain
Sahar. A. Shoman, B. A. Othman
A procedure involving reverse transcription followed by polymerase chain reaction (RT-PCR) with availability of the database sequences (GenBank) provided a sensitive method for the detection or Tobacco mosaic virus- Egyptian strain (TMV-E). Total RNA extract o…
4 Apr 2023- Research Article
Characterization and Molecular Detection of Tomato spotted wilt Tospovirus Infecting Tomato in Egypt
Hayam S. Abdelkader, Gamalat M. Allam, T. A. Moustafa, M. El-Hanunady
Tomato spotted wilt virus was originally isolated from naturally infected tomato fruits. The virus infected a wide host range showing different symptoms varied from local necrosis, systemic necrosis, systemic mosaic yellowing, bronzing, or stunting. The virus…
4 Apr 2023 - Research Article
Human Papillomavirus in Spontaneous Abortion
Enas K. Abo El-Maged, Azza H. El-Salakwy, Safia A. El-Gamal, Gehn Allam
First trimester spontaneously aborted products of conception were obtained from 100 Egyptian women. Placental tissue specimens from 100 women of full term normal vaginal deliveries were used as a control group. Their age ranged from 17-42 years (mean = 29.31).…
4 Apr 2023 - Research Article
Identification of A Virus From Naturally Infected Garlic Plants
S.A. El-Kewey, R.A. Omar, S.A. Sidaros, Samaa Abd El-Khalik
The isolated virus was identified according to host range, symptomatology, differential hosts, virus stability, modes of transmission, and electron microscopy. The virus has a narrow host range. Dilution end point was found to be 10-2. Thermal inactivation poi…
4 Apr 2023 - Research Article
Nucleotide and Amino Acid Sequences Aompatison between Two Strains of barley Stripe Mosaic Hordeivirus (BSMV)
Om-Hashem M. EI-Banna, M. A. S El-Kady, E. A Salama, Salwa N. Zein
Nucleic acid sequence comparison between the Egyptian strain (Gl19) and the American strain (ND 18) of Barley Stripe Mosaic virus (BSMV) revealed a high degree of sequence similarity of γ RNAs comparing to ß RNAs of both strains. On the Other hand,…
4 Apr 2023 - Research Article
Cloning, Expression and Nucleotide Sequence of Coat Protein Gene of an Egyptian Isolate of Potato Virus y Strain NTN Infecting Potato Plants
M. A. Amer, M. H. El-Hammady, H. M. Mazyad, A. A. Shalabyl, F. M. Abo-El-abbas
Reverse transcription- polymerase chain reaction (RT-PCR) amplified product of the expected size of 801 bp was cloned into the Pinpoint Xa-l protein expression vector. The accury of each PCR amplified PVY CP gene was tested by PCR, restriction analysis, and tr…
4 Apr 2023 - Research Article
Molecular Cloning and Expression of the Coat Protein Gene of Plum Pox virus El-Amar Strain in E. coli
M. A. Abou El- Nasr, Kh. A. Dougdoug, Hayam S. Abdelkader, Rehab A. Dawoud
Plum pox potyvirus (PPV), the causal agent of Sharka disease of Prunus inflicts severe crop losses in affected El-Amar area. The virus isolated from EL-Amar apricot trees, was propagated on apricot healthy seedlings. Degenerated oligonucleotide primers were de…
4 Apr 2023 - Research Article
Occurrence of Apple Chlorotic Leaf Spot Trichovirus in Egypt
Salwa N. Zeinl, Hanaa S. Zawam
Apple chlorotic leaf spot virus (ACLSV) isolated from apple trees was successfully transmitted by dagger nematodes (Xiphinema spp) It was transmitted to bait plants grown in soil containing Xiphinema spp. nematodes, The virus was identified according to host r…
4 Apr 2023 - Research Article
Molecular Characterization of Potato Spindle Tuber Viroid Egyptian Isolate
E. K. Allam, Kh. A. El-Dougdoug, M. A. Amer, A. A. Abou-Zaid, S. M. Amin
Potato spindle tuber viroid (PSTVd) was detected in potato plants grown in Egypt using return-polyacrylamide gel electrophoresis (R-PAGE) and molecular techniques. The method involved the extraction of circular nucleic acids followed by electrophoretic analysi…
4 Apr 2023 - Research Article
Prevalence of Herpes simplex Virus in Pregnant Women in Ismailia City
Hanaa H.A. Gomaa, Shimaa A.S. Hasan, Nashwa Harb, Amal H.A. Gomaa, Maha Anani
Herpes simplex virus infections are usually asymptomatic, however infection during pregnancy is life-threatening for pregnant women and their newborns. We aimed to assess both the seroprevalence of herpes simplex virus type 1 and 2 among pregnant women in Isma…
2 Jan 2023DOI 10.17582/journal.jvs/2022/10.1.18.26 
Research ArticleBiological and Molecular Detection of Fig latent trichovirus in Naturally Infected Fig Plants
Hanaa H.A. Gomaa, Dalia Y.Z. Amin, Mona A. Ismail, Khalid A. El-Dougdoug
Different external symptoms of chlorotic to yellowish mottling, mosaic spots and deformation were observed in leaves of fig plants. To evaluate the presence of Fig latent virus (FLV -1) in fig plants. One hundreds fig trees were collected with virus-like sympt…
31 Oct 2022DOI 10.17582/journal.jvs/2022/10.1.8.17
Research ArticleIn Vitro Antiviral Effect of Interferon Produced by Bovine Ephemeral Fever Virus against Rift Valley Fever Virus
Sahar M. Saber, Ahmed M. A. Albehwar, Amani M. Abas, Omaima A.A. Alshamandy, Noha A. El said, Marwa Y. Hamad
This work as an interesting one is a trial to prepare a natural economic antiviral agent against Rift Valley Fever virus which may be able to minimize the virus infection. This study was designed as a preliminary in vitro investigation of the effect of interfe…
31 Oct 2022DOI 10.17582/journal.jvs/2022/10.1.1.7- Articles
Efficacy of the sterile fungus GU23-3 on growth characters of cucumber plants and resistance against Papaya ring spot virus
Shimaa A. Khalaf, Mohsen M. Elsharkawy, Samir A. Sidaros, Shawky A. El Kewey, Ayman F. Omar, Ali Hamed
Background: Papaya ring spot virus (PRSV) is a major threat to the production of cucurbits all over the world. The sterile fungus GU23-3 is a rhizosphere fungus conferring positive effects on plant growth and health.. Objective: The aim of this study is to eva…
20 Aug 2021 - Articles
Induction of Tomato ring spot virus resistant plants using the chemical mutagens (NaN3 and EMS)
Huda S. Darwish, Om-hashem M. El-Banna, Mohamed S. Abbas, Hoda M. Waziri, Maisa A. Awad
Background: Tomato (Solanum lycopersicon) is affected by many biotic and abiotic stresses which became major limiting factors for growth, fruit yield quality and quantity. Tomato ringspot virus (ToRSV) is considered one of the most devastating members of the g…
20 Aug 2021 - Articles
In process metagenomic analysis of LSDV harvest for quality control
Hadeer M. Mossa, Ausama A. Yousif, Emad A. Aboelsoud, Mahmoud El Gamal, Ahmed El- Sanousi
Background: Vaccination against lumpy skin disease is carried out by heterologous sheep pox vaccine (SPPV) giving un-satisfiable results and incomplete protection in vaccinated cattle, since that it was a must to produce a homologues vaccine for LSDV. Methods:…
20 Aug 2021 - Articles
Comparative growth kinetic of Egyptian LSDV isolate in Vero and MDBK cell line
Mohamed M. Mashaly, Ayman H. El-Deeb, Momtaz A. Shahein, Hussein A. Hussein
Background: Lumpy skin disease outbreaks were observed in different governorates of Egypt affecting cattle throughout the year and are still present. The progressive shift of the outbreak in Egypt during 2018 causing severe losses in cattle population despite…
20 Aug 2021 - Articles
Process development of nano emulsion Foot and mouth disease virus vaccine
Soad, E. Elsayed, Mohammed A. Shalaby, Ausama A. Yousef, Abu bakr A. Agor, Sherouk E. Aly
Background: Foot and mouth disease is a highly contagious disease of livestock that is endemic in Egypt with severe economic impact. The main method of control is through vaccination of the livestock with a potent vaccine. Objective: Process development of the…
20 Aug 2021 - Articles
Ultrasonic dispersion of aluminum hydroxide-adjuvanted Rift Valley Fever vaccine enhances formula characteristics and immune response
Shourok E. Aly, Karim Zaki, Soad E. Elsayed, M. Elgamal, Ausama A. Yousef, Mohamed A. Shalaby
Background: Rift Valley Fever (RVF) is a mosquito-borne viral zoonotic disease that is endemic in Africa and the Arabian Peninsula. Outbreaks of RVF resulted in severe losses to the animal industry and major threats to the public health. Control of RVF in Egyp…
20 Aug 2021 - Articles
Lumpy skin disease is circulating among sheep pox vaccinated cattle in Egypt 2017
Akram I. Aboelkhair, Ayman H. El-Deeb, Momtaz A. Shahin, Hussein A. Hussein
Background: Lumpy skin disease is an infectious viral disease caused by Lumpy skin disease virus (LSDV) affecting cattle causing skin lesions. It`s a member of Poxviridea family, genus Capri-pox, and closely related to sheep and goat pox. In Egypt, the protect…
20 Aug 2021 - Articles
Experimental efficacy evaluation of inactivated and live Newcastle disease virus vaccines of genotypes II and VII against wild type virus
Karim M. Selim, Abdullah A. Selim, abdelsattar Arafa, Hussein A. Hussein, Ahmed A. El-Sanousi
Background: Different genotypes of Avian avulavirus 1, also known as Newcastle disease virus (NDV) and formerly known as Avian paramyxovirus 1(APMV-1), are circulating in many parts of the world. Currently, the used vaccine strains (mainly genotypes I and II)…
20 Aug 2021