Articles
308 open-access articles · page 3 of 16
Research ArticleCytological and Molecular Studies of an Egyptian Isolate of Carnation Vein Mottle Potyvirus
Amal Abou El-Ela A, M. A. Amer, Eman A. H. Khatab
Carnation vein mottle virus CarVMV was isolated from infected carnation plants and identified by host-range, serological detection and maintained on Carnation (Dianthus caryophyllus L.) and / or Gompherena globosa. Morphological studies of CarVMV were conducte…
5 Jan 2024- Research Article
Interaction Between Rhizobium Meliloti and Its Specific Viruses in Clover Plants
B. A. Othman, Kh. A. El-Dougdoug, M. H. Abdel Ghaffar, T. F. El-Arabi
Four phages singed as RM1, RM2, RM3, and RM4 specific for Rhizobium meliloti (R meliloti) were isolated from tested rhizosphere soil samples collected from Qalyubia and North Sinai Governorates based on plaque morphology, The extinction spectra of purified pre…
5 Jan 2024 - Research Article
Standardization and Optimization of a Long-Distance PCR Assay for Foot-and-Mouth Disease Virus
M.E. Shawky, A.M. Daoud
Taq DNA polymerase with different concentrations of other thermostable DNA polymerases with 3' to 5' exonuclease activity (PFU and Vent) was used in the amplification of large fragments (2 Kb, 3 CD genes) of foot and mouth disease virus (FMDV). Such long-dista…
5 Jan 2024 - Research Article
Histo-cytopathological Effects of Cucumber Mosaic Cucumovirus on Squash leaves
M. Osman, Kh. El-Dougdoug, E. T. Abd El-Salam, R.M. Taha, R.M. El-Hamidl
An isolate of Cucumber mosaic cucumovirus (CMV) infected cucumber plants was detected by DAS-ELISA and confinned by inoculation of Chenopodium amaranticolor. CMV-isolate was propagated on squash plants var. Eskandarani. After 15 days. systemic symptoms appeare…
5 Jan 2024 - Research Article
Preparation and Evaluation of Inactivated Oil Emulsion Vaccine Against Swollen Head Syndrome in Chickens
Hala M. El-Makaky*•, Sherif, N.A, Fekria El-Bordiny, Taha, M.M
In the present study, inactivated single swollen head syndrome oil emulsion vaccine (SHSV) was prepared and evaluated. SHSV was successfully propagated on the yolk sac of 5-7 days of SPF embryonated chicken eggs. The virus was harvested 48-72 hours post inocul…
4 Jan 2024 - Research Article
In Vitro and In Vivo Evaluation of Inactivated Rift Valley Fever Virus Vaccine
Eman, M. M. Soliman, Taha, M. M, El-Sanousi A, Shalaby M. S. Wasscl et al.
In this study, 5 batches of inactivated rift valley fever (RVF) virus vaccine (ZH501) were evaluated according to Office Internationale Epizooties (OIE) RVF evaluation protocol. The tested batches proved to be sterile and safe when inoculated subcutaneously (S…
4 Jan 2024 - Research Article
Vaccination Trial in Cattle using Mp12 Live Attenuated Rift Valley Fever Virus
Manva F.M. Abdel Monem, Gihan K. Mohammed, Sami A.M, Saber M.S
The present study was aimed to evaluate the live mutagenized RVF-MP12 vaccine in 4 months old calves. The used dose was 5 log10 TCID5Janimal. The safety and potency of the vaccine was followed up as well as the duration of the immunity by ELISA and SNT through…
4 Jan 2024 - Research Article
Cloning and Sequencing of a cDNA Encoding the Coat Protein of an Egyptian Isolate of Pepper Mild Mottle Virus
S.A. Sidaros, S.A. El-Kewey, Hala A. Amin, Eman A.H. Khatab, A.A. Emeran l, Samaa Abd El-Khalilk et al.
A Tobamovirus isolated from pepper plants grown in Egypt has been characterized. Pepper mild mottle Tobamovirus (PMMoV) was isolated from naturally infected pepper plants grown in Kafr El-sheikh Governorate. RT-PCR using a specific non:åegenerate primer…
4 Jan 2024 - Research Article
Studies on the Preparation of an Improved Foot and Mouth Disease oil vaccine
Sonia, A.M, El. Sanousi, A.A, Saber, M.M et al.
This study has been pursued as a trial for preparation and evaluation of an improved Foot and Mouth disease oil vaccine by using four different adjuvants which are: Montanide ISA 206, Quil A saponine, Ginseng extract and Montanide IMS 3015. These vaccines were…
4 Jan 2024 - Research Article
Isolation and Characterization of PI-3 Virus from Sheep and Goats
JEHAN A. M. GAFER.' HUSSEIN, H.A, REDA I.M
225 nasal swab samples were collected from diseased sheep and goats for the isolation of PI-3 virus. The samples were taken from diseased animal at seven different Governorates Kaffer el sheikh, Alexandria, EL Behaira, Port Said, Demiatta El-Qalubia and Giza t…
4 Jan 2024 - Research Article
Medicinal Plants Used in the traditional Treatment of Several Viral Diseases in Bangladesh
Mazharul A. M, Md. S. Sarwar, M. Nurullah, Keshab K. Adhikary I, M. Rahmatullah
Viral fevers, measles, chicken pox, and rabies are fairly common occurrences throughout Bangladesh. Since the population of the country is predominantly rural without proper access to modern medical facilities, they rely on traditional medical practitiofiers (…
4 Jan 2024 - Research Article
Comparative Molecular Analysis of Isolated Strains of Highly Pathogenic Avian Influenza Virus H5N1 during 2006 and 2007
Madbouly, H.M, Orkhan, M.H, Nagwa El-khoty
Highly pathogenic avian influenza (Al) H5N1 strains have been isolated during February 2006 from infected chicken located at El-Qanater El-Khiria center, Qaluobia, Eypt. These isolates were molecularly characterized and compared with those H5Nl strains is…
4 Jan 2024 - Research Article
Synthesis of the Bovine Rotavirus Major Neutralization Antigen (VP7) Using A Baculovirus Expression System
El-Sabagh, I.M, Hussein, H.A, Amer, H.M et al.
The present study reports the successful production of the major neutralization antigen (VP7) of bovine romvirus Nebraska calf diarrhea virus (NCDV) strain in insect cells. The full-length DNA copies of RNA segment 9 (coding for VP7 protein) of NCDV was insert…
4 Jan 2024 - Research Article
Isolation and Antigenic and Molecular Characterization of G10 of Group A Rotavirus in Camel
Eman, Abo Hatab, Hussein, El-Sabagh IM, Saber
We are here reporting the successful Isolation and characterization of GIO serotype of group A rotaviruses from fecal samples collected from camel farms suffering from diarrhea in Alexandria and Esmalia governorates. After preparation of fecal samples and inoc…
4 Jan 2024 - Research Article
Differentiation among Three Egyptian Isolates of Citrus Psorosis Virus
El-Dougdoug, Kh.A. t, S.A. Ghaza, A.A. Mousa, H. Fahmyj, A.R. sofy
Three Egyptian isolates of Citrus psorosis virus (CPsV-EG), namely ARC, TB and TN were obtained from citrus cvs. Grapefruit, Balady and Navel, respectively. These isolates Were differed in some of their external symptoms. The CPsV-EG isolates were detect…
4 Jan 2024 - Research Article
WHO Global Influenza Program Surveillance and Network, 2005; (www.oie.int). Outbreak of highly pathogenic avian influenza (Al) H5N1 in poultry in Egypt 2006
Madbouly, H.M, Orkhan, M.H, Nagwa El-khoty
Avian influenza outbreak was firstly appeared in poultry reared in backyards or roofs of homes in Qaluobia & Giza governorates as many notifications were received by the General Organization of Veterinary Services (GOVSs) in February 2006. The disease was…
4 Jan 2024 - Research Article
Re-Emergence of Very Virulent IBDV in Egypt
Ashraf M. Metwally, Ausama A. Yousif*k, Iman B. Walaa A. Attia M. Samy, Ismail M. Reda
Infectious bursal disease (IBD) serotype 1 viruses continue to cause major economic losses in the Egyptian poultry industry despite the implementation of intensive vaccination programs. A recent increase in IBD related mortality in vaccinated farms prompted th…
4 Jan 2024 - Research Article
Immune Response to a Live Attenuated Chicken Anemia Virus (CAV) Vaccine. The Absence of Virus Shedding
Hanan M.M. Elzahed, Abd EL-Moneim A.A, Afaf A. Khedr, Elham A. Elabiare, Waleed S. Abd El-Latif, Reda M.I
An assessment of the performance of a live attenuated (CAV) vaccine following administration to broiler breeder hens in eight houses at Il weeks of age. Antibodies to (CAV) in adult hens and maternally derived antibodies to (CAV) in progeny chicks were a…
4 Jan 2024 - Research Article
Study on the Infection of Zoo Birds by Highly Pathogenic H5N1 Avian Influenza Virus
Arafa, El-Kanawaty, Hassan, M.K, Anwar, H.K et al.
From February to June 2006 an epidemic of highly pathogenic avian influenza (HPAI) virus Of subtype H5N1 affected all commercial poultry sectors as well as rural and backyard level in Egypt. This outbreak also was extended to include the zoo birds In Giza zoo.…
4 Jan 2024 - Research Article
Effect of Silnultaneous Vaccination with the Bivalent Foot and Mouth Vaccine and Bovine Ephemeral Fever Vaccine on the Immune Response OF Cattle
Attyat, M. Kotb, Abeer, E. Mansour, Naglaa, I. Aly et al.
The present study was aimed to evaluate the vaccination of cattle against bovine ephemeral fever (BEF) and bivalent foot and mouth disease (FMD) strain O and strain A. so fifteen calves, six month old, were vaccinated singly and simultaneously and two calves w…
4 Jan 2024