In the present study, inactivated single swollen head syndrome oil emulsion vaccine (SHSV) was prepared and evaluated. SHSV was successfully propagated on the yolk sac of 5-7 days of SPF embryonated chicken eggs. The virus was harvested 48-72 hours post inoculation. The titre of the virus reached 1062 EID50ml. One hundred and fifty chickens were divided into 3 equal groups. The first group was vaccinated with the prepared SHS vaccine, 2nd group two was vaccinated with imported SHS vaccine, while the third group was kept as non-vaccinated control. Evaluation of the prepared vaccine was done by measuring both cell-mediated and humoral immunity. The results of both T-lymphocyte transformation and ELISA tests revealed that the prepared vaccine induced good protection and has good potentiality for use in chicken in the Egyptian poultry industry.