Articles
308 open-access articles · page 2 of 16
Research ArticlePersistence of Maternal Antibodies in Calves Born to Dams Naturally Infected with Bovine Ephemeral Fever Virus
S. A. Khalil, M. El-Sayed, M. M. El-Fayomy, H. Y. Hassan, A. Zaghawa
Bovine ephemeral fever virus (BEFV) is an arthropod born rhabdovirus which causes disabling febrile infection among cattle and water buffalo. The disease is characterized by inflammation of mesodermal tissue as achieved by muscular shivering, stiffness, lamene…
5 Jan 2024- Research Article
Preparation and Evaluation of Anti-Rabbit IgY Peroxidase and FITC Conjugates
N. A. Sherif, Lamiaa M. Omar, S. M. Shafei, Elham A. El-Ebiary
In this investigation, a trial for preparation of anti-rabbit immunoglobulin-Y IgY peroxidase and fluorescein isothiocyanate (FITC) conjugates was conducted. Rabbit IgG was separated and injected into specific pathogen free (SPF) laying hens, from which their…
5 Jan 2024 - Research Article
Identification and Elimination Treatment of Prunus necrotic Ring Spot Virus (PNRSV) in Rose
Amal Abou El-Ela
Prunus necrotic ring spot Ilarvirus (PNRSV), was isolated from rose shrubs during the survey of rose plantations in Orman Garden, showing Ilarvirus-like symptoms. To identify the causal virus, the plants were tested by enzyme-linked immunosorbent assay using a…
5 Jan 2024 - Research Article
Cytological and Molecular Studies of an Egyptian Isolate of Carnation Vein Mottle Potyvirus
Amal Abou El-Ela A, M. A. Amer, Eman A. H. Khatab
Carnation vein mottle virus CarVMV was isolated from infected carnation plants and identified by host-range, serological detection and maintained on Carnation (Dianthus caryophyllus L.) and / or Gompherena globosa. Morphological studies of CarVMV were conducte…
5 Jan 2024 - Research Article
The Influence of Gibberellic Acid (GA) and Indole Acetic Acid (IAA) on The Production of Local Lesions on Nicotiana sylvestris Plants Infected with Tomato Mosaic Tobamovirus (ToMV)
E.F. Mohamed, I.M. Sabra
Tomato mosaic disease caused by Tomato mosaic (ToMV) is widespread wherever tomato is grown ToMV affects plants and yields. Virus was isolated and identified on the base of symptomatology on tomato plants, host range, diagnostic hosts, physical ptoperties, mod…
5 Jan 2024 - Research Article
An Inhibitory Activity of Propolis Extract against Faba Bean Mottle Bromovirus (BBMV)
E.F. Mohamedl, A.A. Owayss
A Bromovirus was isolated from naturally infected faba bean plants in Fayoum Governorate. The virus was identified as Faba bean mottle Virus (BBMV) according to symptomatology on Faba bean plants, host range, diagnostic hosts, virus stability and ELISA detecti…
5 Jan 2024 - Research Article
Occurrence of Rose Colour Break Tobamovirus in Egypt
Amal Abo El-Ela Ahmed, Eman A.H. Khatab, M.S. Shafie
Rose colour break virus was detected in naturally infected rose growing in Orman Garden for ornamental plants, Giza Governorate and it was found to be widely spread in rose fields. The virus was transmitted mechanically and by grafting (chipbudding) but not by…
5 Jan 2024 - Research Article
Production of FMDV ELISA Kit for Antibodies Detection in Animals Using Native Strain with Production of Reference Hyper-immune Serum in Guinea Pigs
M. E. Shawky, A. M. Daoud
The objective of the present study was to evaluate an enzyme linked immunosorbent assay (ELISA) based kit for detecting foot and mouth disease (FMD) antibodies using a native FMDV strain (O1. Aga 93). Results were compared to those of serum neutralization test…
5 Jan 2024 - Research Article
Isolation and Characterization of a Pseudomonas aeruginosa Bacteriophage from Agriculture Drain Water
Amina A. II, M.K. Amin
Five bacteriophages infecting Pseudomonas aeruginosa strain were isolated from agricultural drain water of Zagazig city. One of them designated as PAI has been characterized. The ability of phage PAI to form plaques with strain PAOI was examined. Two kinds of…
5 Jan 2024 - Research Article
Phylogenetic Characterization of Some Bovine viral diarrhea viruses in Egypt
A. A. El-Kholy, S. Vilcek, A. M. Daoud
Sixty-seven buffy coat specimens, obtained from clinically suspected cattle at farms located in Belbees. El-Sharquia, were screened for BVDV. Only 9/67 of the tested specimens (13%) were BVDV positive by reverse transcription-polymerase chain reaction (RT-PCR)…
5 Jan 2024 - Research Article
Purification, Serology and Prevalence of Broad bean stain Comovirus (BBSV) and Cowpea Aphid Borne Mosaic Potyvirus (CABMV)
S. A. Sidaros, S. A. EL-Kewey, Eman A. H. khattab, M. M. ELsharkawy
BBSV and CABMV were purified from faba bean and cowpea plants, respectively using two different methods. The UV absorption spectrum had maxima and minima at 260 and245 nm, respectively for the two viruses. The absorbance ratios of A and A260/280 of the combine…
5 Jan 2024 - Research Article
Detection and Characterization of Apple Chlorotic Leaf Spot Virus (ACLSV) Isolate from peach orchards in Egypt
A.A.Farrag, I.A.M. Ibrahim, H.M. Mazyad
Apple Chlorotic Leaf Spot virus (ACLSV) was isolated from symptomless peach trees and then identified with a specific antiserum (Sanofi comp) using Double Antibody Sandwich ELISA (DAS-ELISA) and Dot Immunobinding Assay (DIBA). Survey was carried out during 200…
5 Jan 2024 - Research Article
Isolation and Characterization of a Pseudomonas Putida Bacteriophages
Mayada A. Abd Elgalell, B.A Othman, Th. Radwan, Amal S.M. Abo-Sinna
Two phage isolates for Pseudomonas putida were isolated from soil cultivated with potato by the enrichment culture technique and signed as PPI and PP2. The phages were propagated and purified by polyethylene glycol, 6000-sodium dextran sulfate 500 two phase sy…
5 Jan 2024 - Research Article
Comparative Study on Two Thrips Transmitted Viruses Tomato spotted wilt virus (TSWV) and Iris yellow spot virus (IYSV) as a Tospovirus
Manal A. El-Shazly, A. s. 2 Abdel Wahab, Salwa N. Zein
Biological, biochemical and serological comparison between Tomato spotted wilt virus (TSWV) and Iris yellow spot virus (IYSV) a Tospovirus were studied These two viruses were isolated from Vinca rosae (L). G. Don and onion (Allium cepa L.), respectively. The o…
5 Jan 2024 - Research Article
Virus Elimination from Infected Garlic Plants Using Different Techniques
S.A. Sidaros, R.A. Omar, S.A. El-Kewey l, Samaa Abd El-Khalik
Production of garlic virus free plant was attempted by means of chemotherapy, meristem-tip culture, and thermotherapy techniques. Three different meristem sizes (5, 3 and 1 mm long) were excised from three garlic cultivars (Chinese. Italian and Balady). Merist…
5 Jan 2024 - Research Article
Physiological and Histopathological Changes in Potato Virus X and Potato virus Y- Infected Potato Plants
Hanaa H.A.Gomaa, A. F. Moustafal, Kh.A.El-Dougdoug, A.A. Abou-Zeid, S. Y.M. Mahmoud
Potato viruses X and Y were used for study the effect of the two viruses on the metabolism of potato plants. It was found that detectable decrease or dry matter, total nitrogen, tuber Starch, number of starch granules, and total protein. The nucleic acid conte…
5 Jan 2024 - Research Article
Standardization and Optimization of a Long-Distance PCR Assay for Foot-and-Mouth Disease Virus
M.E. Shawky, A.M. Daoud
Taq DNA polymerase with different concentrations of other thermostable DNA polymerases with 3' to 5' exonuclease activity (PFU and Vent) was used in the amplification of large fragments (2 Kb, 3 CD genes) of foot and mouth disease virus (FMDV). Such long-dista…
5 Jan 2024 - Research Article
Alterations of Immunologic Responses of Calves Experimentally Infected with Virulent Either Type I or 2 Non-Cytopathogenic Strains of Bovine Viral Diarrhea Virus
Ahmed Abd El-Samie H. Ali
Bovine viral diarrhea virus (BVDV) is a worldwide pathogen infecting cattle. Eleven Holstein calves between 6-8 months of age that were seronegative to BVDV were divided into three groups (n=4 for NY-1, n=4 for 890 and n=3 control). Fibroblast cells were prepa…
5 Jan 2024 - Research Article
Population Fluctuations of Aphids in Relation to Virus Disease Incidence in Egyptian Faba Bean Fields
Mohga M. El-Tahlawey, A. Mandour, AE Aboulata
Infection induced by both Faba bean necrotic virus (FBNYV) and mosaic causing viruses were inspected during Oct. 2003 to Apr. 2004 on Faba bean fields grown in Qalyubia governorate. Population density of seven different aphid species on faba bean was arranged…
5 Jan 2024 - Research Article
A New Whitefly Transmitted Geminivirus Infecting Tomato Plants in Egypt
A-New-Whitefly-Transmitted-Geminivirus
Whitefly transmitted Geminiviruses cause severe damage to different crops in Egypt. Geminiviruses often occur in mixed infections, and it is difficult to diagnose such mixtures by biological properties. Two types of yellow leaf curl symptoms were collected fro…
5 Jan 2024